miR-377 inhibition enhances the survival of trophoblast cells via upregulation of FNDC5 in gestational diabetes mellitus
| Autor: | Anqin Wu, Luo Shi, Dana Li, Hua Zhaozhao, Ting Cao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system diseases Cell Type 2 diabetes cell survival mir-377-3p 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Internal medicine Medicine fndc5 business.industry Cell growth Trophoblast General Medicine medicine.disease FNDC5 trophoblast cells gestational diabetes mellitus Gestational diabetes 030104 developmental biology medicine.anatomical_structure Endocrinology Apoptosis 030220 oncology & carcinogenesis business Research Article |
| Zdroj: | Open Medicine, Vol 16, Iss 1, Pp 464-471 (2021) Open Medicine |
| ISSN: | 2391-5463 |
| Popis: | Gestational diabetes mellitus (GDM) is a metabolic dysregulation closely related to both obesity and type 2 diabetes; however, the molecular mechanism underlying GDM is still unclear. The purpose of this study was to investigate the effects of microRNA-377 (miR-377-3p) and fibronectin type III domain containing 5 (FNDC5) in regulating the cell growth of trophoblasts under high glucose (HG) conditions during the development of GDM. Serum miR-377-3p was upregulated and positively correlated with fasting blood glucose level in GDM patients. miR-377-3p downregulation increased the cell vitality and suppressed the cell apoptosis of HG-treated HTR-8/SVneo and BeWo cells. Using TargetScan prediction, luciferase assay, and western blot, it was found that miR-377-3p could target FNDC5 and suppress its expression. However, FNDC5 downregulation abolished the effect of miR-377-3p inhibitor in HTR-8/SVneo cells. Together, miR-377 is a potential target for GDM biomarker, which promotes cell growth and suppresses cell apoptosis, partly through the upregulation of FNDC5. |
| Databáze: | OpenAIRE |
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