Assessment of neurovascular dynamics during transient ischemic attack by the novel integration of micro-electrocorticography electrode array with functional photoacoustic microscopy

Autor: Eddie Tung Wee Tan, You Yin Chen, Wen Li, Aishwarya Bandla, Wai Hoe Ng, Yen-Yu Ian Shih, Lun-De Liao, Yu Hang Liu, Ki Yong Kwon, Nitish V. Thakor, Stacey Tan, Ji Min Ling, Hsin Yi Lai
Rok vydání: 2015
Předmět:
Male
Middle Cerebral Artery
Time Factors
Microscopy
Acoustic
Electroencephalography
Functional photoacoustic microscopy (fPAM)
lcsh:RC321-571
Transient ischemic attack (TIA)
Photoacoustic Techniques
Rats
Sprague-Dawley
Evoked Potentials
Somatosensory
medicine.artery
medicine
Electrode array
Animals
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
Electrocorticography
Cerebral Cortex
Blood Volume
medicine.diagnostic_test
Chemistry
Lasers
Equipment Design
medicine.disease
Neurovascular bundle
Electric Stimulation
Electrodes
Implanted
Alpha Rhythm
Disease Models
Animal
Delta Rhythm
Neurology
Ischemic Attack
Transient
Somatosensory evoked potential
Cerebrovascular Circulation
Micro-electrocorticography (μECoG)
Middle cerebral artery
Neurovascular function
Functional Photoacoustic Microscopy
Neuroscience
Photothrombotic ischemia
Biomedical engineering
Zdroj: Neurobiology of Disease, Vol 82, Iss, Pp 455-465 (2015)
ISSN: 0969-9961
Popis: This study developed a novel system combining a 16-channel micro-electrocorticography (μECoG) electrode array and functional photoacoustic microscopy (fPAM) to examine changes in neurovascular functions following transient ischemic attack (TIA) in rats. To mimic the pathophysiology of TIA, a modified photothrombotic ischemic model was developed by using 3 min illumination of 5 mW continuous-wave (CW) green laser light focusing on a distal branch of the middle cerebral artery (MCA). Cerebral blood volume (CBV), hemoglobin oxygen saturation (SO2), somatosensory evoked potentials (SSEPs) and alpha-to-delta ratio (ADR) were measured pre- and post-ischemia over a focal cortical region (i.e., 1.5 × 1.5 mm2). Unexpectedly, the SO2, peak-to-peak amplitude (PPA) of SSEPs and ADR recovered and achieved levels greater than the baseline values at the 4th hour post-ischemia induction without any intervention, whereas the CBV value only partially recovered. In other words, transient ischemia led to increased neural activity when the relative CBV was reduced, which may further compromise neural integrity or lead to subsequent vascular disease. This novel μECoG-fPAM system complements currently available imaging techniques and represents a promising technology for studying neurovascular coupling in animal models.
Databáze: OpenAIRE
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