General acid and base catalysis by phosphate in Peroxyoxalate Chemiluminescence
| Autor: | Glalci Alves de Souza, Wilhelm J. Baader, Ana Paula Fileno dos Santos, Mônica Melchioretto de Medeiros Peixoto |
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| Rok vydání: | 2016 |
| Předmět: |
Bioanalysis
010405 organic chemistry Inorganic chemistry General Chemistry 010402 general chemistry Phosphate CATÁLISE 01 natural sciences Peroxyoxalate Dimethoxyethane 0104 chemical sciences Catalysis law.invention chemistry.chemical_compound chemistry law Light emission Acid–base reaction Chemiluminescence |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | The peroxyoxalate reaction, one of the most efficient chemiluminescent transformations, is widely utilized in analytical and bioanalytical assays. Although the mechanism of this transformation has been extensively studied, it is still not yet fully clarified. Furthermore, no detailed mechanistic studies have been performed in aqueous media, highly important for analytical applications. The light emission kinetic behaviour of the peroxyoxalate reaction is studied here in phosphate buffered binary 1,2-dimethoxyethane/water mixtures at different pH values using three oxalic ester derivatives. The results indicate that conditions can be found where the chemiluminescent perhydrolysis is predominant over dark ester hydrolysis; the reactions are subject to general acid and base catalysis by phosphate ions; are reasonably reproducible, although, generally fast. However, chemiluminescence emission quantum yields are much lower than in organic solvents. |
| Databáze: | OpenAIRE |
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