| Autor: |
Hnin Yu Lwin, Yukari Aoki-Nonaka, Aoi Matsugishi, Naoki Takahashi, Takumi Hiyoshi, Koichi Tabeta |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Archives of Oral Biology. 142:105497 |
| ISSN: |
0003-9969 |
| DOI: |
10.1016/j.archoralbio.2022.105497 |
| Popis: |
This study aimed to clarify the antibacterial mechanism and antibiofilm effect of soybean-derived peptide BCBS-11 against periodontopathic bacteria.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of BCBS-11 against Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum), and Streptococcus mitis (S. mitis) were determined for the antibacterial mechanism. The effect of BCBS-11 on membrane permeability and depolarization activity were investigated using propidium iodide (PI) staining and 3, 3'-dipropylthiadicarbocyanine iodide (DiSCThe MIC and MBC indicated the bactericidal activity of BCBS-11 against P. gingivalis and F. nucleatum. The PI staining revealed that BCBS-11 disrupted the bacterial membrane integrity. The DiSCBCBS-11 inhibits the monospecies and multispecies biofilm formation of P. gingivalis and F. nucleatum, and their bactericidal activity results from membrane disruption. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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