Effect of chemically modified tetracycline-8 (CMT-8) on hematology, blood chemistry, cytokines and peripheral blood lymphocyte subsets of healthy dogs
| Autor: | Ángel Sainz, Mercedes García-Sancho, José Julio de Lucas, Manuel Ignacio San Andres, María Dolores San Andrés, Casilda Rodríguez, Fernando Rodríguez-Franco, Alejandra Villaescusa, Beatriz Agulla |
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| Rok vydání: | 2020 |
| Předmět: |
Erythrocyte Indices
Male congenital hereditary and neonatal diseases and abnormalities medicine.medical_specialty medicine.drug_class Antibiotics Mean corpuscular hemoglobin Pharmacology Immune system Dogs Internal medicine medicine Animals RNA Messenger Hematology General Veterinary medicine.diagnostic_test business.industry Complete blood count Lymphocyte Subsets nervous system diseases Anti-Bacterial Agents Blood chemistry Tetracyclines Peripheral blood lymphocyte Toxicity Cytokines Female business Blood Chemical Analysis |
| Zdroj: | Research in veterinary science. 136 |
| ISSN: | 1532-2661 |
| Popis: | Tetracyclines are antibiotics widely used in human and veterinary medicine. Effects on the immune system and inflammatory response, including effects on blood leukocytes proliferation and function and in cytokines synthesis, have been described. Chemically modified tetracyclines (CMT) have lost their antimicrobial activity, but maintain these other properties. This study analyzes the effect of chemically modified tetracycline-8 (CMT-8) on the evolution of complete blood count, blood chemistry, the mRNA expression of selected cytokines and peripheral blood lymphocyte subpopulations distribution in healthy dogs. CMT-8 at a dose of 10 mg/kg once daily was administered per os to six healthy dogs. A control group of five healthy dogs, living in the same conditions than dogs treated with CMT-8, received placebo with an identical therapeutic regimen. When given at the doses used in this study, no side effects of CMT-8 were detected, suggesting a good tolerance and a limited toxicity of the drug. Dogs treated with CMT-8 showed a gradual increase in mean corpuscular hemoglobin. The administration of CMT-8 in healthy dogs did not affect blood mRNA expression of IFN-γ, TNFα, IL-4, IL-6, IL-10, IL-12 p40 and IL-13. However, the lymphocytes expressing class II MHC on their surface decreased during the first two weeks of CMT-8 treatment and subsequently increased for the next three months. Considering the absence of antimicrobial properties of the drug, the effects of CMT-8 detected in this study seem to be unrelated to the classical antimicrobial activity attributed to tetracyclines. |
| Databáze: | OpenAIRE |
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