Comparison of serum sodium and chloride results for Flame IV-Auto Analyzer II, SMAC, Ektachem 400, and Nova 4 clinical analyzer systems
| Autor: | Susan J. Matte, C. Jacklyn, Deborah I. Ryder, M. Moss, L.C. Dymond, M.A. MacAulay, Charman L. Cousins |
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| Rok vydání: | 1988 |
| Předmět: |
Spectrum analyzer
Autoanalysis Chemistry Sodium Clinical Biochemistry Analytical chemistry chemistry.chemical_element Reference range General Medicine Nova (laser) AutoAnalyzer Chloride Chlorides Evaluation Studies as Topic medicine Potentiometry Serum electrolytes Humans medicine.drug Total protein |
| Zdroj: | Clinical biochemistry. 21(3) |
| ISSN: | 0009-9120 |
| Popis: | We determined serum Na+ and Cl− results using Technicon's Flame IV-Auto Analyzer II (FLIV/AAII) system and Kodak's Ektachem 400 clinical analyzer. Our objective was to determine whether Na+ and Cl− results from these analyzers were sufficiently similar to report to clinicians without reference to the system used for the determination. Method precision of the two systems for Na+ results was comparable; whereas Ektachem 400 Cl− results were more imprecise than those determined using the FLIV/AAII, Ektachem Na+ results showed lower correlation with the FLIV/AAII (r = 0.890) and Cl− results were more highly correlated (r = 0.960). When Kodak's newly developed equi-transferant electrolyte reference fluid (ETRF) was used with generation 4 Na+ slides and generation 1 Cl− slides the largest difference observed was 7.0 mmol/L for both Na+ and Cl− results. Using Kodak calibrators and the manufacturer's operational conditions for the Ektachem 400, we observed that a considerable number of sample results for both Na+ and Cl− did not agree within 3.0 mmol/L of the FLIV/AAII values. To corroborate our finding, we also analyzed serum Na+ and Cl− using a Technicon Sequential Multiple Analyzer + Computer (SMAC) system and a Nova 4 + 4 Clinical Analyzer (Nova). We conclude that flame emission systems and direct ion specific electrode systems do not yield comparable Na+ and Cl− results even when total protein and triglyceride concentrations of the samples are within reference ranges. Furthermore, Ektachem 400 Na+ results were only comparable with those obtained by the FLIV/AAII system when Kodak's latest generation of slides (GEN 4) and ERF (GEN 4) were used with alignment of the two systems using the slope and intercept of a least squares regression line as recommended by Kodak. Therefore, unless the alignment is carried out using these materials, clinicians must be made aware of the system used for the determination of serum electrolytes in order to apply an appropriate reference range. |
| Databáze: | OpenAIRE |
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