Protease immobilization on cellulose monoacetate/chitosan-blended nanofibers
| Autor: | Tuba Avci, Elif Demirkan, Yakup Aykut |
|---|---|
| Přispěvatelé: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Uludağ Üniversitesi/Mühendislik Fakültesi/Tekstil Mühendisliği Bölümü., Demirkan, Elif, Avcı, Tuba, Aykut, Yakup, ABI-4472-2020 |
| Rok vydání: | 2017 |
| Předmět: |
Glutaraldehyde activation
Polymers and Plastics medicine.medical_treatment Nanofibers Chitin 02 engineering and technology 01 natural sciences Differential scanning calorimeters Industrial and Manufacturing Engineering Physical adsorption method Chitosan chemistry.chemical_compound Chemical Engineering (miscellaneous) Chemical analysis Enzyme activity Catalysts Fourier transform infrared spectroscopy Spinning (fibers) Cellulose acetates Chemical activation 021001 nanoscience & nanotechnology Microspheres Electrospinning Enzymes Fibers Thermogravimetric analyzers 0210 nano-technology Scanning electron microscopy Immobilized Enzymes Candida Rugosa Materials science Immobilized enzyme Cellulose acetate Materials Science (miscellaneous) Sodium Immobilized enzyme activity chemistry.chemical_element 010402 general chemistry Sodium hydroxide Differential scanning calorimetry medicine Enzyme immobilization Cellulose Membranes Protease Acetate Alpha-amylase Thermoanalysis Lipase immobilization Electrospinning process 0104 chemical sciences chemistry Chemical engineering Nanofiber Nanoparticles Materials science textiles |
| Zdroj: | Journal of Industrial Textiles. 47:2092-2111 |
| ISSN: | 1530-8057 1528-0837 |
| Popis: | Chitosan-blended cellulose monoacetate nanofibers were prepared through electrospinning process. Neat nanofibers and their sodium hydroxide-treated analogs were used as support surfaces for protease immobilization via physical adsorption method. Morphologies of the nanofibers were observed with a scanning electron microscopy. Chemical analyses were conducted with Fourier transform infrared spectroscopy, and thermal analyses were carried out with differential scanning calorimeter and thermogravimetric analyzer. Immobilized enzyme activities were measured by using casein substrate. In order to test the stability of immobilized enzymes, the tests were repeated until the immobilized enzyme activity was leveled off. The results reveal that well uniform cellulose monoacetate/chitosan nanofibers were obtained, and nanofiber structures are transformed from rounded to more flattened morphology after enzyme activation test. Glutaraldehyde activation has positive effect on sodium hydroxide-treated samples, and the highest immobilization yield as about 83% was observed for glutaraldehyde-treated cellulose monoacetate/chitosan samples. Sodium hydroxide treatment before glutaraldehyde activation shows the best results for protease immobilization on cellulose monoacetate and cellulose monoacetate/chitosan nanofibers. Operational stability increases after sodium hydroxide treatment and glutaraldehyde activation. Glutaraldehyde activation effectively increased the cycle number after sodium hydroxide treatment and about 20% of enzyme activity was still retained after seven cycles at cellulose monoacetate/chitosan samples. This percentage is higher at pure cellulose monoacetate nanofibers than cellulose monoacetate/chitosan nanofibers and measured around 33.5%. British Association for Psychopharmacology Fırat Üniversitesi |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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