The response of two polar monocyte subsets to inflammation
| Autor: | Evgeny Karpulevich, Polina Vishnyakova, Anastasiya S. Poltavets, A. Makarov, Evgeniya Kananykhina, V. Vtorushina, A. Maznina, Gennadiy T. Sukhikh, Timur Fatkhudinov, A.V. Lokhonina, Andrey Elchaninov, L. Mikhaleva, Sergey I. Kovalchuk |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Lipopolysaccharides Proteomics Lipopolysaccharide CD14 medicine.medical_treatment Lipopolysaccharide Receptors Inflammation RM1-950 CD16 Monocytes 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system Phagocytosis Polarization medicine Macrophage Humans Pharmacology Innate immune system Chemistry Macrophages Receptors IgG NF-kappa B Computational Biology General Medicine Flow Cytometry Healthy Volunteers Cell biology 030104 developmental biology Cytokine 030220 oncology & carcinogenesis Culture Media Conditioned Cytokines RNA Female Therapeutics. Pharmacology B7-2 Antigen medicine.symptom Transcriptome |
| Zdroj: | Biomedicine & Pharmacotherapy, Vol 139, Iss, Pp 111614-(2021) |
| ISSN: | 1950-6007 |
| Popis: | Macrophages are a central component of innate immunity that play an important role in the defense of the organism. Macrophages are highly plastic and are activated by interaction with other cells and environmental factors. In this work, we study the effect of lipopolysaccharide on macrophages derived from the two most polar (CD14+ and CD16+ monocytes) as well as the intermediate subset of blood monocytes from healthy donors and assess what happens to the subset most prone to polarization on the transcriptomic and proteomic level. It has been shown that, according to primary pro-inflammatory polarization markers, their cytokine profile, and their phagocytic activity, macrophages derived from CD14+ monocytes exhibit higher sensitivity to inducers of pro-inflammatory polarization. Flow cytometry analysis revealed increased levels of CD86, while secretome analysis demonstrated significant increase of pro-inflammatory and anti-inflammatory cytokines observed in CD14+-derived macrophages, as compared to CD16+-derived macrophages in conditioned media. Assessment of the transcriptome and proteome of CD14+-derived macrophages with further bioinformatic analysis identified the most significant differences after polarization towards the pro-inflammatory phenotype. Immune-, membrane-, IFN-γ-, cytokine-, and defense-associated pathways were found significantly prevalent, while downregulated pathways were represented by RNA binding-, housekeeping-, exocytosis-, intracellular transport-, peptide and amide metabolic-related signaling. This data could be useful for macrophage-based cell therapeutics of cancer, as it provides additional background for the manipulation of donor monocytes intended for back transplantation. |
| Databáze: | OpenAIRE |
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