Evaluation of Eight Rapid Screening Tests for Acute Leptospirosis in Hawaii
| Autor: | Alan R. Katz, Paul V. Effler, Harry Y. Domen, David M. Sasaki, Henry Y. Higa, April K. Bogard |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
medicine.medical_specialty Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Predictive Value of Tests Leptospira Internal medicine Direct agglutination test medicine Humans Mass Screening Leptospirosis Mass screening biology business.industry Bacteriology Dipstick medicine.disease biology.organism_classification Antibodies Bacterial Latex fixation test Immunoglobulin M Specimen collection Predictive value of tests Acute Disease Immunology Reagent Kits Diagnostic business Latex Fixation Tests |
| Zdroj: | Journal of Clinical Microbiology. 40:1464-1469 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.40.4.1464-1469.2002 |
| Popis: | Leptospirosis is a major public health problem throughout the world. Clinical recognition of leptospirosis is challenging, and the definitive serologic diagnostic assay, the microscopic agglutination test, is time-consuming and difficult to conduct. Various serologic screening tests have been developed, but their performance among ill persons in the United States has not been established. Eight screening tests were compared using 379 serum samples obtained in 1998 and 1999 from a series of 236 patients (33 with confirmed infection). The median number of days between illness onset and specimen collection was 9. The overall sensitivity, by specimen, for each test was as follows: indirect hemagglutination assay (MRL Diagnostics, Cypress, Calif.), 29%; INDX Leptospira Dip-S-Tick (PanBio InDx, Inc., Baltimore, Md.), 52%; Biognost IgM IFA test (Bios GmbH Labordiagnostik, Gräfelfing, Germany), 40%; Biolisa IgM ELISA (Bios GmbH, Labordiagnostik), 48%; Leptospira IgM ELISA (PanBio Pty Ltd., Brisbane, Australia), 36%; SERION ELISA classic Leptospira (Institut Virion•Serion GmbH, Würzburg, Germany), 48%; LEPTO Dipstick(Organon-Teknika, Ltd., Amsterdam, The Netherlands), 34%; Biosave latex agglutination test (LATEX; Bios GmbH Labordiagnostik), 86%. Test specificity ranged from 85 to 100% among all tests except LATEX, for which the specificity was significantly lower, at 10%. Test sensitivity was particularly low ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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