Naturally-occurring right terminal hairpin mutations in three genotypes of canine parvovirus (CPV-2a, CPV-2b and CPV-2c) have no effect on their growth characteristics
| Autor: | Jianlou Zhang, Ying Liu, Ji Xi, Weiquan Liu, Peiran Li, Yongle Yu, Jigui Wang, Xiaomei Zhang |
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| Rok vydání: | 2019 |
| Předmět: |
China
Cancer Research Genotype Parvovirus Canine viruses Cat Diseases medicine.disease_cause Cell Line Parvoviridae Infections 03 medical and health sciences Plasmid Virology medicine Animals 030304 developmental biology Viral Structural Proteins Genetics 0303 health sciences Mutation Virulence biology 030306 microbiology Canine parvovirus Epithelial Cells Sequence Analysis DNA biology.organism_classification Reverse Genetics Infectious Diseases Terminal (electronics) DNA Viral Cats |
| Zdroj: | Virus Research. 261:31-36 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2018.12.007 |
| Popis: | We have isolated 4 naturally-occurring strains of CPV in mainland China and have identified them as CPV-2, 2a, 2b and 2c genotypes according to their VP2 sequences which also revealed substitutions within their right terminal regions. To determine if these substitutions affected the growth characteristics of the 4 strains, we constructed plasmids based on their genomic sequences minus their right terminal sequences, with the latter replaced by a single right terminal region. Analysis of rescued recombinants showed that the substitutions within their natural right termini had no significant effect on their growth characteristics. |
| Databáze: | OpenAIRE |
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