Defined Geldrop Cultures Maintain Neural Precursor Cells
| Autor: | Carsten Werner, Steffen Vogler, Marcus Binner, Silvana Prokoph, Gerd Kempermann, Mikhail V. Tsurkan, Uwe Freudenberg |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
drug effects [Neural Stem Cells] lcsh:Medicine High density metabolism [Neural Stem Cells] Cell morphology chemistry [Hydrogels] Article metabolism [rho-Associated Kinases] Polyethylene Glycols Mice 03 medical and health sciences In vivo drug effects [Cell Adhesion] Precursor cell Neurosphere methods [Cell Culture Techniques] Animals drug effects [Cell Self Renewal] cytology [Neural Stem Cells] lcsh:Science Protein kinase A Glycosaminoglycans rho-Associated Kinases Multidisciplinary chemistry [Glycosaminoglycans] Chemistry drug effects [Cell Differentiation] lcsh:R pharmacology [Hydrogels] Hydrogels In vitro Cell biology chemistry [Polyethylene Glycols] 030104 developmental biology Intracellular signaling cascade lcsh:Q ddc:600 |
| Zdroj: | Scientific reports 8(1), 8433 (2018). doi:10.1038/s41598-018-26417-1 Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-26417-1 |
| Popis: | Distinct micro-environmental properties have been reported to be essential for maintenance of neural precursor cells (NPCs) within the adult brain. Due to high complexity and technical limitations, the natural niche can barely be studied systematically in vivo. By reconstituting selected environmental properties (adhesiveness, proteolytic degradability, and elasticity) in geldrop cultures, we show that NPCs can be maintained stably at high density over an extended period of time (up to 8 days). In both conventional systems, neurospheres and monolayer cultures, they would expand and (in the case of neurospheres) differentiate rapidly. Further, we report a critical dualism between matrix adhesiveness and degradability. Only if both features are functional NPCs stay proliferative. Lastly, Rho-associated protein kinase was identified as part of a pivotal intracellular signaling cascade controlling cell morphology in response to environmental cues inside geldrop cultures. Our findings demonstrate that simple manipulations of the microenvironment in vitro result in an important preservation of stemness features in the cultured precursor cells. |
| Databáze: | OpenAIRE |
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