Reduced CXCL1 production by endogenous IL-37 expressing dendritic cells does not affect T cell activation
| Autor: | Wilco P. Pulskens, Charles A. Dinarello, Luuk B. Hilbrands, M.A.H. Bakker-van Bebber, J. van der Vlag, Mihai G. Netea, M. Kouwenberg, Laura E. Diepeveen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Physiology Chemokine CXCL1 T-Lymphocytes lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] Cell Communication Lymphocyte Activation Mice White Blood Cells Spectrum Analysis Techniques Animal Cells Immune Physiology Medicine and Health Sciences Cytotoxic T cell Cells Cultured Staining Innate Immune System Multidisciplinary T Cells Chemistry Genetically Modified Organisms Cell Staining Cell Differentiation respiratory system Flow Cytometry Recombinant Proteins Cell biology medicine.anatomical_structure Spectrophotometry Cytokines Engineering and Technology Medicine Cytophotometry Cellular Types Genetic Engineering Cell activation Research Article Biotechnology Naive T cell Immune Cells T cell Science Primary Cell Culture Immunology Antigen-Presenting Cells Mice Transgenic Bioengineering Cytotoxic T cells Research and Analysis Methods medicine Animals Humans Cell Proliferation CD86 Blood Cells Genetically Modified Animals Cell growth Biology and Life Sciences Dendritic Cells Cell Biology Dendritic cell Molecular Development Renal disorders Radboud Institute for Molecular Life Sciences [Radboudumc 11] Specimen Preparation and Treatment Immune System Renal disorders Radboud Institute for Health Sciences [Radboudumc 11] CD80 Interleukin-1 Developmental Biology |
| Zdroj: | PLoS ONE, Vol 16, Iss 5, p e0251809 (2021) PLoS One, 16 PLoS One, 16, 5 PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Contains fulltext : 235331.pdf (Publisher’s version ) (Open Access) The dendritic cell (DC)-derived cytokine profile contributes to naive T cell differentiation, thereby directing the immune response. IL-37 is a cytokine with anti-inflammatory characteristics that has been demonstrated to induce tolerogenic properties in DC. In this study we aimed to evaluate the influence of IL-37 on DC-T cell interaction, with a special focus on the role of the chemokine CXCL1. DC were cultured from bone marrow of human IL-37 transgenic (hIL-37Tg) or WT mice. The phenotype of unstimulated and LPS-stimulated DC was analyzed (co-stimulatory molecules and MHCII by flow cytometry, cytokine profile by RT-PCR and ELISA), and T cell stimulatory capacity was assessed in mixed lymphocyte reaction. The role of CXCL1 in T cell activation was analyzed in T cell stimulation assays with anti-CD3 or allogeneic DC. The expression of the co-stimulatory molecules CD40, CD80 and CD86, and of MHCII in LPS-stimulated DC was not affected by endogenous expression of IL-37, whereas LPS-stimulated hIL-37Tg DC produced less CXCL1 compared to LPS-stimulated WT DC. T cell stimulatory capacity of LPS-matured hIL-37Tg DC was comparable to that of WT DC. Recombinant mouse CXCL1 did not increase T cell proliferation either alone or in combination with anti-CD3 or allogeneic DC, nor did CXCL1 affect the T cell production of interferon-γ and IL-17. Endogenous IL-37 expression does not affect mouse DC phenotype or subsequent T cell stimulatory capacity, despite a reduced CXCL1 production. In addition, we did not observe an effect of CXCL1 in T cell proliferation or differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |