Living cell imaging by far-field fibered interference scanning optical microscopy

Autor: Joël Chevrier, Serge Huant, Hervé Guillou, Wilfrid Schwartz, Jochen Fick, Catherine Villard, Jean-Baptiste Decombe
Přispěvatelé: Nano-Optique et Forces (NOF), Institut Néel (NEEL), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Thermodynamique et biophysique des petits systèmes (TPS)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Materials science
02 engineering and technology
01 natural sciences
law.invention
Interference microscopy
Mice
Optics
Optical microscope
law
0103 physical sciences
Animals
Fiber Optic Technology
Microscopy
Interference

010306 general physics
Fiber optics imaging
Cells
Cultured

Neurons
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics]
Microscopy
Confocal

Quantitative Biology::Neurons and Cognition
business.industry
Scanning confocal electron microscopy
Scanning microscopy
Equipment Design
Fibroblasts
Image Enhancement
021001 nanoscience & nanotechnology
Dark field microscopy
Atomic and Molecular Physics
and Optics

Equipment Failure Analysis
Scanning ion-conductance microscopy
Digital holographic microscopy
Near-field scanning optical microscope
Scanning tunneling microscope
0210 nano-technology
business
Zdroj: Optics Express
Optics Express, Optical Society of America-OSA Publishing, 2011, 19 (3), pp.2702--2710. ⟨10.1364/OE.19.002702⟩
ISSN: 1094-4087
DOI: 10.1364/OE.19.002702⟩
Popis: International audience; We report on the imaging of biological cells including living neurons by a dedicated fibered interferometric scanning optical microscope. The topography and surface roughness of mouse fibroblasts and hippocampal neurons are clearly revealed. This straightforward far-field technique allows fast, high resolution observation of samples in liquids without lengthy alignment procedures or costly components.
Databáze: OpenAIRE