Simultaneous quantification of carbohydrates and metabolites in multicomponent fermentation broths by means of high-performance thin-layer chromatography
| Autor: | Paola Pedrini, Tatiana Bernardi, Elena Tamburini, Stefania Costa, Irene Rugiero |
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| Rok vydání: | 2017 |
| Předmět: |
Clinical Biochemistry
Xylose Xylitol 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Arabitol Planar chromatography Overpressured layer chromatography Fermentation Carbohydrates analysis Metabolites analysis C. tropicalis spp Planar chromatography Organic chemistry Monosaccharide Overpressured layer chromatography High performance thin layer chromatography Sugar chemistry.chemical_classification C. tropicalis spp Chromatography 010405 organic chemistry Metabolites analysis 010401 analytical chemistry Ambientale food and beverages 0104 chemical sciences chemistry Fermentation Sorbitol Carbohydrates analysis |
| Zdroj: | JPC - Journal of Planar Chromatography - Modern TLC. 30:170-174 |
| ISSN: | 1789-0993 0933-4173 |
| Popis: | Quantification of carbohydrates and metabolites in fermentation processes can be considered as key factor in determining yield and productivity for a better understanding of the microbial behavior under different conditions. The main aim of the present study was to develop and set up analytical methods for detecting complex sugar and/or metabolite mixtures in fermentation broth based on high-performance thin-layer chromatography (HPTLC). HPTLC is a fast and accurate method of separating complex mixtures, based on planar development. The proposed methods involved the separations of a mixture of monosaccharides (glucose, xylose, arabinose, and rhamnose) deriving from delignification and hydrolysis of hazelnut shells and the corresponding sugar alcohols (xylitol, arabitol, and sorbitol) obtained by fermentations of Candida tropicalis spp., a yeast able to ferment aldoses to produce sugar alcohols. HPTLC methods were set up on simple chamber development and using instrumental techniques like overpressured lay... |
| Databáze: | OpenAIRE |
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