Expression of ICAM-1 in implanted primary and metastatic squamous cell carcinomas in rats

Autor: Xinwei Li, Rehana Sultana Lovely, Tomoo Sawada, Yu Hsueh Wang, Nobuko Tokuda, Yoshikazu Hayatsu, Fumihiko Shinozaki, Yoshihisa Fujikura, Fukumoto T
Rok vydání: 1997
Předmět:
CD4-Positive T-Lymphocytes
Cancer Research
Pathology
medicine.medical_specialty
Mesenchyme
CD8 Antigens
CD8-Positive T-Lymphocytes
Pathology and Forensic Medicine
Metastasis
Immunoenzyme Techniques
Mesoderm
Random Allocation
MHC class I
medicine
Tumor Cells
Cultured
Animals
Coloring Agents
Hematoxylin
Lymph node
Fluorescent Dyes
ICAM-1
biology
Macrophages
Histocompatibility Antigens Class II
Antibodies
Monoclonal
Rats
Inbred Strains
medicine.disease
Intercellular Adhesion Molecule-1
Rats
Tongue Neoplasms
Gene Expression Regulation
Neoplastic
medicine.anatomical_structure
Otorhinolaryngology
Epidermoid carcinoma
Lymphatic Metastasis
Monoclonal
CD4 Antigens
Lip Neoplasms
biology.protein
Carcinoma
Squamous Cell
Periodontics
Eosine Yellowish-(YS)
Mouth Neoplasms
Lymph Nodes
Oral Surgery
Facial Neoplasms
Immunostaining
Neoplasm Transplantation
Zdroj: Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 26(8)
ISSN: 0904-2512
Popis: FF6 tumor cells are derived from a spontaneous rat squamous cell carcinoma (SCC) which originally arose in the facial skin of a DA rat. In this study, FF6 tumor cells were implanted into rat oral mucosa to establish an ex vivo metastatic model. We analyzed the expression of intercellular cell adhesion molecule-1 (ICAM-1) in the implanted primary and metastatic FF6 tumors by immuno-staining with a monoclonal antibody (mAb) against ICAM-1. The implanted primary FF6 cells showed strong expression of ICAM-1, whereas the tumor cells of metastatic lesions showed weak or negative expression of ICAM-1. By immunostaining with mAb OX6, a number of MHC class II-positive macrophages were detected in tumor mesenchyme and surrounding the metastatic foci. These results suggested that the local immune reaction in the lymph node influenced the expression of ICAM-1 on tumor cells, and that MHC class II-positive macrophages may play a role in transplanted tumor growth and metastases.
Databáze: OpenAIRE
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