Cytomorphological Features of Hyperchromatic Crowded Groups in Liquid-Based Cervicovaginal Cytology: A Single Institutional Experience
| Autor: | Lee, Youngeun, Lee, Cheol, Park, In Ae, An, Hyoung Jin, Kim, Haeryoung |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Histology Population Pathology and Forensic Medicine 03 medical and health sciences 0302 clinical medicine Cytology medicine lcsh:Pathology Outpatient clinic liquid-based cytology education hyperchromatic crowded groups education.field_of_study business.industry Cervicovaginal cytology medicine.disease cervical neoplasms Squamous intraepithelial lesion 030104 developmental biology 030220 oncology & carcinogenesis Liquid-based cytology Adenocarcinoma Liquid based Original Article business lcsh:RB1-214 |
| Zdroj: | Journal of Pathology and Translational Medicine, Vol 53, Iss 6, Pp 393-398 (2019) Journal of Pathology and Translational Medicine |
| ISSN: | 2383-7845 2383-7837 |
| Popis: | Background Hyperchromatic crowed groups (HCGs) are defined as three-dimensional aggregates of crowded cells with hyperchromatic nuclei, and are frequently encountered in cervicovaginal liquid-based cytology (LBC). Here, we aimed to examine the prevalence of HCGs in cervicovaginal LBC and the cytomorphological characteristics of various epithelial cell clusters presenting as HCGs. Methods We first examined the prevalence of HCGs in a "routine cohort" of LBC cytology (n=331), consisting of all cervicovaginal LBCs accessioned over 3 days from outpatient clinics (n=179) and the screening population (n=152). Then we examined a second "high-grade epithelial cell abnormalities (H-ECA) cohort" (n=69) of LBCs diagnosed as high-grade squamous intraepithelial lesion (HSIL), squamous cell carcinoma (SCC), or adenocarcinoma during 1 year. Results HCGs was observed in 34.4% of the routine cohort and were significantly more frequent in the epithelial cell abnormality category compared to the non-neoplastic category (p=.003). The majority of HCGs represented atrophy (70%). Of the 69 histologically confirmed H-ECA cases, all contained HCGs. The majority of cases were HSIL (62%), followed by SCC (16%). Individually scattered neoplastic cells outside the HCGs were significantly more frequent in SCCs compared to glandular neoplasia (p=.002). Despite the obscuring thick nature of the HCGs, examining the edges and the different focal planes of the HCGs and the background were helpful in defining the nature of the HCGs. Conclusions HCGs were frequently observed in cervicovaginal LBC and were mostly non-neoplastic; however, neoplastic HCGs were mostly high-grade lesions. Being aware of the cytomorphological features of different HCGs is important in order to avoid potential false-negative cytology interpretation. |
| Databáze: | OpenAIRE |
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