Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes
| Autor: | Paolo Costantino, G. Risuleo, R. A. Schilperoort, Maria Luisa Mauro, Gioacchino Micheli, Paul J. J. Hooykaas |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1981 |
| Předmět: |
Genetics
biology Base Sequence Agrobacterium Virulence Nucleic Acid Hybridization DNA Restriction Enzymes biology.organism_classification Microbiology Restriction enzyme Ti plasmid Plasmid Sequence homology Species Specificity Electron microscopy Agrobacterium rhizogenes plasmids Molecular Biology T-DNA Binary system Plasmids Rhizobium |
| Zdroj: | Plasmid 5 (1981): 170–182. doi:10.1016/0147-619X(81)90018-4 info:cnr-pdr/source/autori:Costantino, Paolo A.; Mauro, Maria Luisa; Micheli, Gioacchino; Risuleo, Gianfranco; Hooykaas, Paul J J; Schilperoort, Rob A./titolo:Fingerprinting and sequence homology of plasmids from different virulent strains of Agrobacterium rhizogenes/doi:10.1016%2F0147-619X(81)90018-4/rivista:Plasmid (Print)/anno:1981/pagina_da:170/pagina_a:182/intervallo_pagine:170–182/volume:5 |
| Popis: | Several wild-type virulent strains of Agrobacterium rhizogenes, belonging to both biotypes 1 and 2, were analyzed for plasmid content. All the strains were found to harbor at least one large plasmid, of a size comparable to that of A. tumefaciens Ti plasmids as determined by electron microscopy. The plasmids were characterized by restriction endonuclease finger-printing and compared for sequence homology by the Southern blot-hybridization technique. Despite the diversity of the restriction cleavage patterns, the plasmids of the various strains share rather extensive sequence homology. All of the biotype 1 organisms analyzed were shown to harbor one common plasmid. |
| Databáze: | OpenAIRE |
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