The buds of Oscarella lobularis (Porifera): a new convenient model for sponge cell and developmental biology
| Autor: | Carole Borchiellini, Christian Marschal, Cedric Matthews, Morgan Dutilleul, Alexander V. Ereskovsky, Laura Fierro-Constain, Florent Marschal, Emilie Le Golf, Emmanuelle Renard, Sandrine Chenesseau, André Le Bivic, Amélie Vernale, Nicolas Brouilly, Dominique Massey-Harroche, Caroline Rocher, Nina Séjourné |
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| Přispěvatelé: | Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut de recherche pour le développement [IRD] : UMR237-Aix Marseille Université (AMU)-Avignon Université (AU) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Cnidaria 0303 health sciences biology Phylum [SDV]Life Sciences [q-bio] biology.organism_classification 010603 evolutionary biology 01 natural sciences Amphimedon queenslandica Homoscleromorpha 03 medical and health sciences Sponge Ctenophora Evolutionary biology Placozoa 14. Life underwater Developmental biology 030304 developmental biology |
| Popis: | The comparative study of the four non-bilaterian phyla (Cnidaria, Placozoa, Ctenophora, Porifera) should provide insights into the origin of bilaterian traits. Except for Cnidaria, present knowledge on the cell biology and development of these animals is so far limited. Non-bilaterian models are needed to get further into cell architecture and molecular mechanisms.Given the developmental, histological, ecological and genomic differences between the four sponge classes, we develop a new sponge model: the buds of theOscarella lobularis(class Homoscleromorpha). This experimental model supplements the two other most famous sponge modelsAmphimedon queenslandicaandEphydatia muelleri, both belonging to the class Demospongiae.Budding is a natural and spontaneous asexual reproduction mean, but budding can be triggeredin vitroensuring availability of biological material all year long. We provide a full description of buds, from their formation to their development into juveniles. Their transparency enables fluorescent and live imaging, and their abundance allows for experimental replicates. Moreover, regeneration and cell reaggregation capabilities provide interesting experimental morphogenetic contexts. The numerous techniques now mastered on these buds make it a new suitable sponge model.Summary statementStudying sponge biology is needed to understand the evolution of metazoans. We developed a new model suitable for experimental biology that allows to study morphogenetic processes with modern tools. |
| Databáze: | OpenAIRE |
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