Improved culture-based detection and quantification of Burkholderia pseudomallei from soil
| Autor: | Trinh Thanh Trung, Eylin Topfstedt, Ivo Steinmetz, Vanaporn Wuthiekanun, André Göhler, Direk Limmathurotsakul, Adrian Hetzer, Sharon J. Peacock |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Burkholderia pseudomallei Melioidosis Soil test Population structure Colony Count Microbial Dispersion (geology) Microbiology medicine Humans Soil Microbiology Sodium Deoxycholate biology Public Health Environmental and Occupational Health General Medicine Thailand bacterial infections and mycoses medicine.disease biology.organism_classification Infectious Diseases bacteria Female Parasitology Soil microbiology Bacteria |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 105:346-351 |
| ISSN: | 0035-9203 |
| DOI: | 10.1016/j.trstmh.2011.03.004 |
| Popis: | Environmental surveillance of the Gram-negative soil bacterium Burkholderia pseudomallei, the aetiological agent of melioidosis, is important in order to define human populations and livestock at risk of acquiring the infection. This study aimed to develop a more sensitive method for the detection of B. pseudomallei from soil samples in endemic areas compared with the currently used culture method based on soil dispersion in water. We report the development of a new protocol that involves soil dispersion in a polyethylene glycol (PEG) and sodium deoxycholate (DOC) solution to increase the yield of viable B. pseudomallei from soil samples. Comparative testing of soil samples from Northeast Thailand covering a wide range of B. pseudomallei concentrations demonstrated a significantly higher recovery (P |
| Databáze: | OpenAIRE |
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