Are minichromosomes valid model systems for DNA replication control? Lessons learned fromEscherichia coli
| Autor: | David B. Bates, Tsuneaki Asai, Tokio Kogoma, Erik Boye |
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| Rok vydání: | 1998 |
| Předmět: |
DNA Replication
Genetics Models Genetic Semiconservative replication Origin Recognition Complex Eukaryotic DNA replication Chromosomes Bacterial Biology Pre-replication complex Origin of replication Microbiology Article DNA-Binding Proteins Viral Proteins Control of chromosome duplication SeqA protein domain Minichromosome maintenance Escherichia coli Origin recognition complex Molecular Biology |
| Zdroj: | Molecular Microbiology. 29:671-675 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1046/j.1365-2958.1998.00901.x |
| Popis: | Initiation of chromosome replication is a key event in the life cycle of any organism. Little is known, however, about the regulatory mechanisms of this vital process. Conventionally, the initiation mechanism of chromosome replication in microorganisms has been studied using plasmids in which an origin of chromosome replication has been cloned, rather than using the chromosome itself. The reason for this is that even bacterial chromosomes are so large that biochemical and genetic manipulations become difficult and cumbersome. Recently, the combination of flow cytometry and genetic methods, in which modifications of the replication origin are systematically introduced onto the chromosome, has made possible detailed studies of the molecular events involved in the control of replication initiation in Escherichia coli. The results indicate that requirements for initiation at the chromosomal origin, oriC, are drastically different from those for initiation at cloned oriC. |
| Databáze: | OpenAIRE |
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