Metallothionein Induction in the RTH-149 Cell Line as an Indicator for Heavy Metal Bioavailability in a Brackish Environment: Assessment by RT-Competitive PCR
| Autor: | Baruch Rinkevich, Jacob Douek, Moshe Tom, I. Kamer |
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| Rok vydání: | 2003 |
| Předmět: |
Carcinoma
Hepatocellular Health Toxicology and Mutagenesis Biological Availability chemistry.chemical_element Biology Toxicology Cell Line Metals Heavy Animals Bioassay Metallothionein Water Pollutants Water pollution Pollutant Cadmium Brackish water Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms Fishes General Medicine Pollution Salinity chemistry Enzyme Induction Environmental chemistry Biological Assay Seawater |
| Zdroj: | Archives of Environmental Contamination and Toxicology. 45:0086-0091 |
| ISSN: | 1432-0703 0090-4341 |
| Popis: | Estuarine and marine near-shore environments are often subjected to heavy metal pollution. We establish a bioassay using the quantitative evaluation of metallothionein (MT) transcript in the fish hepatoma cell line, RTH-149, as a tool for detecting heavy metal pollution in brackish-marine water containing other pollutants in addition to heavy metals. RT-competitive polymerase chain reaction was used for the quantitative evaluation of the transcript in absolute units. Cadmium was used as a model pollutant to optimize two parameters of the assay: exposure periods (24, 96, 144 h) and salinity (0%, 25%, 50%, 75%, 100% sea water). Results revealed that salinity at or below 25% sea water at an exposure period of 144 h are the preferable conditions for detecting MT mRNA levels for in vitro assays employed on water samples from highly polluted brackish habitats. |
| Databáze: | OpenAIRE |
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