A native, highly active Tc1/mariner transposon from zebrafish (ZB) offers an efficient genetic manipulation tool for vertebrates
| Autor: | Chengyi Song, Shuheng Chan, Zoltán Ivics, Bo Gao, Csaba Miskey, Dan Shen, Zhongxia Guan, Cai Chen, Yali Wang, Yatong Sang, Xiaoyan Wang, Ferenc Müller |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Transposable element
AcademicSubjects/SCI00010 Mutagenesis (molecular biology technique) Computational biology Biology Genome Gentechnologie Transposon 03 medical and health sciences Mice 0302 clinical medicine Genetics Animals Humans Enhancer Gene Zebrafish Molecular Biology 030304 developmental biology 0303 health sciences Hep G2 Cells biology.organism_classification Enhancer Elements Genetic Regulatory sequence Mutagenesis DNA Transposable Elements Functional genomics 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | Nucleic acids research, 49(4):2126-2140 Nucleic Acids Research |
| Popis: | New genetic tools and strategies are currently under development to facilitate functional genomics analyses. Here, we describe an active member of the Tc1/mariner transposon superfamily, named ZB, which invaded the zebrafish genome very recently. ZB exhibits high activity in vertebrate cells, in the range of those of the widely used transposons piggyBac (PB), Sleeping Beauty (SB) and Tol2. ZB has a similar structural organization and target site sequence preference to SB, but a different integration profile with respect to genome-wide preference among mammalian functional annotation features. Namely, ZB displays a preference for integration into transcriptional regulatory regions of genes. Accordingly, we demonstrate the utility of ZB for enhancer trapping in zebrafish embryos and in the mouse germline. These results indicate that ZB may be a powerful tool for genetic manipulation in vertebrate model species. |
| Databáze: | OpenAIRE |
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