Molecular and biological characterization of a replication competent human immunodeficiency type 2 (HIV-2) proviral clone
| Autor: | Eva Maria Fenyö, G Biberfeld, L Jagodzinski, M Bosch, Kathleen Fargnoli, J Albert, A. De Rossi, Genoveffa Franchini, R C Gallo, F Giombini |
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| Rok vydání: | 1989 |
| Předmět: |
Genes
Viral T-Lymphocytes viruses Restriction Mapping Clone (cell biology) Fluorescent Antibody Technique Biology Molecular cloning Transfection Virus Replication Virus Cell Line law.invention Restriction map law Humans Cloning Molecular Gene Multidisciplinary virus diseases Provirus Virology Molecular biology Blotting Southern Microscopy Electron Viral replication DNA Viral HIV-2 Recombinant DNA Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 86:2433-2437 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | We obtained complete genomic clones of human immunodeficiency virus type 2 (HIV-2) from the DNA of the neoplastic human cell line HUT 78 freshly infected with a HIV-2 isolate, strain SBL6669. The recombinant phage DNA was transfected into the lymphocytes of CD4-positive HUT 78 cell line to test the replication competence of the proviral DNA. One genomic clone, designated HIV-2SBL/ISY, yielded retroviral particles after a few weeks of culture of the transfected cells. The HIV-2SBL/ISY clone contained a complete provirus and cellular flanking sequence. We obtained the DNA sequence of the provirus and compared it with the published sequence of two other HIV-2 isolates. The degree of variability among HIV-2 isolates is comparable to that observed among African HIV-1 isolates sequenced to date. Immunologically, HIV-2SBL/ISY is similar to the parental virus (HIV-2SBL6669) but differs in the envelope transmembrane protein that is truncated (gp32-34) in the parental virus and not in HIV-2SBL/ISY (gp41). Both the parental and the cloned viruses are infectious and cytopathic for some human T-cell lines, induce syncytia, and infect a human macrophage cell line (U937) in vitro. The availability of a biologically active HIV-2 clone provides the means to study the role and interaction of HIV-2 genes in vitro as well as to assess the functional similarities among HIV-1 and HIV-2 genes. Since HIV-2SBL/ISY cloned virus infects fresh peripheral blood T cells from Rhesus macaques in vitro and infects the same animal in vivo, its use in animals may represent a model for functional study of viral genes in vivo as well as for development of experimental approaches to prevent and cure retroviral infection in humans. |
| Databáze: | OpenAIRE |
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