Galactose-containing polysaccharides from the human pathogens Sporothrix schenckii and Ceratocystis stenoceras
| Autor: | Luiz R. Travassos, Lucia Mendonça-Previato, P. A. J. Gorin |
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| Rok vydání: | 1980 |
| Předmět: |
Magnetic Resonance Spectroscopy
Immunology Polysaccharide Methylation Microbiology chemistry.chemical_compound Galactomannan Ascomycota Polysaccharides Amylose Humans Sporothrix schenckii Carbon Radioisotopes skin and connective tissue diseases chemistry.chemical_classification biology Sporothrix Galactose Galactan bacterial infections and mycoses biology.organism_classification Fungal antigen Infectious Diseases chemistry Biochemistry Parasitology Research Article |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Galactose-containing polysaccharides from three strains of Sporothrix schenckii and one strain of Ceratocystis stenoceras were isolated, and their structures were paritally characterized by chemical analysis and 13C-nuclear magnetic resonance spectroscopy (13C-NMR). S. schenckii polysaccharide preparations from all strains were not precipitated by Fehling solution and contained galactomannan (or a mixture of galactan and galactomannan), amylose, and minor amounts of rhamnomannan. C. stenoceras polysaccharide contained galactomannan and a smaller proportion of amylose. Conventional chemical techniques and 13C-NMR spectroscopy showed that the structures of the two preparations were closely related. The core of the galactomannan consisted principally of nonreducing end units and 2-O-, 2,6-di-O-, and perhaps 2,3-di-O-substituted alpha-D-mannopyranosyl units. The core was substituted by beta-D-galactofuranosyl chains; the units are interlinked (1 leads to 6). 13C-NMR evidence shows that the alpha-D-mannopyranosyl units are substituted in the two positions by the beta-D-galactofuranosyl residues. Galactomannans present at the cell surface of S. schenckii represent other potential fungal antigens in addition to the already recognized rhamnomannans and their peptide complexes. |
| Databáze: | OpenAIRE |
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