Purification of Rickettsia rickettsi by density-gradient zonal centrifugation
| Autor: | R. K. Gerloff, W. D. Bickel, W. R. Brown, R L Anacker, L. A. Thomas, R. E. Mann |
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| Rok vydání: | 1974 |
| Předmět: |
Sucrose
food.ingredient Density gradient Rocky Mountain spotted fever Immunology Extraembryonic Membranes Rickettsia rickettsii Cell Count Chick Embryo Applied Microbiology and Biotechnology Microbiology Mice L Cells food Dry weight Yolk Genetics medicine Animals Centrifugation Yolk sac Molecular Biology Antigens Bacterial Chromatography biology Complement Fixation Tests Embryonated General Medicine medicine.disease biology.organism_classification Centrifugation Zonal Microscopy Electron Rickettsia medicine.anatomical_structure embryonic structures |
| Zdroj: | Canadian Journal of Microbiology. 20:1523-1527 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m74-238 |
| Popis: | Rocky Mountain spotted fever rickettsiae, cultivated either in yolk sacs of embryonated chicken eggs or in mouse L cells, were purified by centrifugation through a linear sucrose density gradient in a zonal rotor. Under the conditions of the experiments the rickettsiae banded at a density of about 1.21. Intact organisms with what appeared to be large translucent capsules were found in this band. The degree of purity was high because yolk sac contamination was not detected in rickettsial suspensions derived from embryonated eggs, and little extraneous material was observed by electron microscopy in suspensions obtained from either host. About 4 × 108 rickettsial particles were recovered from each gram of yolk sac and 2 × 1010 particles from each gram of L cells, a 50-fold difference. Each milligram dry weight of purified rickettsiae contained about 1 × 1010 particles. |
| Databáze: | OpenAIRE |
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