Formation and relevance of 5-hydroxymethylfurfural in bioactive subcritical water extracts from olive leaves
| Autor: | José A. Ferragut, María Castro-Puyana, Alejandro Cifuentes, Miguel Herrero, Elena Ibáñez, Lourdes Rocamora-Reverte |
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| Přispěvatelé: | Ministerio de Educación y Ciencia (España), Comunidad de Madrid, Ministerio de Ciencia e Innovación (España), European Commission |
| Rok vydání: | 2012 |
| Předmět: |
5-hydroxymethylfurfural
Chromatography Antioxidant medicine.medical_treatment Extraction (chemistry) Water extraction Mass spectrometry Subcritical water extraction chemistry.chemical_compound Maillard reaction symbols.namesake chemistry High pressure symbols medicine Antiproliferative HMF SWE Hydroxymethylfurfural Food Science |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0963-9969 |
| Popis: | Although subcritical water extraction (SWE) has already shown its great potential for the attainment of natural bioactive extracts, concerns still remain on possible unexpected reactions that can arise during the extraction process, usually taking place at high pressure and temperature. It is already well-known that different components might be formed during the SWE extraction protocol due e.g. to Maillard reaction, which can improve the bioactivity of the obtained extracts. On the other hand, the formation of other compounds derived from these reactions, such as 5-hydroxymethylfurfural (HMF), has raised some concerns, mainly related to its safety. In this work, the formation of HMF during subcritical water extraction, at different conditions, from olive leaves has been monitored by using liquid chromatography with mass spectrometry (LC-MS) and diode array detection (LC-DAD). The possible influence of this compound in the overall antioxidant and antiproliferative activities against colon cancer cells has been also studied. Results showed an increase of HMF formation when increasing the extraction temperature, being the maximum concentration achieved at 200 °C (3.17 μg. HMF/mg extract); nevertheless, the HMF contained in the olive leave extracts did not influence the antioxidant capacity or the antiproliferative activity of the natural extracts, thus demonstrating the safety of the SWE process. © 2012 Elsevier Ltd. This work has been financed by AGL2008-05108-C03-01 and AGL2011-29857-C03-01 (Ministerio de Educacion y Ciencia), CSD2007-00063 FUN-CFOOD (Programa CONSOLIDER-INGENIO 2010) and by ALIBIRD, S2009/AGR-1469 (Comunidad de Madrid) projects. M.H. would like to thank MICINN for a “Ramón y Cajal” research contract. M.C.P. thanks MICINN for her “Juan de la Cierva” contract. |
| Databáze: | OpenAIRE |
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