Steroid hormone pathways coordinate developmental diapause and olfactory remodeling in Pristionchus pacificus
| Autor: | Elissa A. Hallem, Ray L. Hong, Navonil Banerjee, Reinard M. Villalon, Heather R Carstensen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Developmental and Behavioral Genetics
Receptors Steroid Steroid hormone receptor medicine.medical_treatment ved/biology.organism_classification_rank.species Olfaction Diapause Dauer larva Pheromones 03 medical and health sciences Rhabditida 0302 clinical medicine Genetics medicine Animals Host-Seeking Behavior Caenorhabditis elegans 030304 developmental biology Neurons 0303 health sciences biology ved/biology Cholestenes fungi Gene Expression Regulation Developmental Helminth Proteins biology.organism_classification Phenotype Coleoptera Smell Steroid hormone Pristionchus pacificus Genetic Loci Larva Mutation Odorants 030217 neurology & neurosurgery Metabolic Networks and Pathways |
| Zdroj: | Genetics |
| ISSN: | 1943-2631 |
| Popis: | Developmental and behavioral plasticity allow animals to prioritize alternative genetic programs during fluctuating environments. Behavioral remodeling may be acute in animals that interact with host organisms, since reproductive adults and the developmentally arrested larvae often have different ethological needs for chemical stimuli. To understand the genes that coordinate the development and host-seeking behavior, we used the entomophilic nematode Pristionchus pacificus to characterize dauer-constitutive mutants (Daf-c) that inappropriately enter developmental diapause to become dauer larvae. We found two Daf-c loci with dauer-constitutive and cuticle exsheathment phenotypes that can be rescued by the feeding of Δ7-dafachronic acid, and that are dependent on the conserved canonical steroid hormone receptor Ppa-DAF-12. Specifically at one locus, deletions in the sole hydroxysteroid dehydrogenase (HSD) in P. pacificus resulted in Daf-c phenotypes. Ppa-hsd-2 is expressed in the canal-associated neurons (CANs) and excretory cells whose homologous cells in Caenorhabditis elegans are not known to be involved in the dauer decision. While in wildtype only dauer larvae are attracted to host odors, hsd-2 mutant adults show enhanced attraction to the host beetle pheromone, along with ectopic activation of a marker for putative olfactory neurons, Ppa-odr-3. Surprisingly, this enhanced odor attraction acts independently of the Δ7-DA/DAF-12 module, suggesting that Ppa-HSD-2 may be responsible for several steroid hormone products involved in coordinating the dauer decision and host-seeking behavior in P. pacificus. |
| Databáze: | OpenAIRE |
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