Protective efficacy of four heat-shock proteins as recombinant vaccines against photobacteriosis in Asian seabass (Lates calcarifer)
| Autor: | Pei-Chi Wang, Ta-Chih Cheng, Trung H.M. Pham, Shih-Chu Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
0301 basic medicine Aquatic Science Biology Microbiology Fish Diseases 03 medical and health sciences Immune system Antigen Heat shock protein Animals Environmental Chemistry Heat-Shock Proteins Vaccines Synthetic Photobacterium 04 agricultural and veterinary sciences General Medicine Hsp90 Hsp70 Blot 030104 developmental biology Photobacterium damselae Bacterial Vaccines 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Bass Antibody Gram-Negative Bacterial Infections |
| Zdroj: | Fish & Shellfish Immunology. 111:179-188 |
| ISSN: | 1050-4648 |
| Popis: | Photobacterium damselae subsp. piscicida (Phdp) is the causative agent of photobacteriosis in marine fish and is responsible for huge losses to marine aquaculture worldwide. Efforts have been made to develop a vaccine against this disease. Heat-shock proteins (HSPs) are a family of proteins that are ubiquitous in cellular life. Bacteria produce elevated levels of HSPs as a survival strategy when exposed to stressful environments in a host during infection. This group of proteins are also important antigens that can induce both humoral and cellular immune responses. In this study, four HSPs of Phdp, HSP90, HSP33, HSP70, and DnaJ, were selected for cloning and recombinant expression. Western blotting with rabbit anti-Phdp helped identify rHSP70 and rHSP33 as immunogenic proteins. Asian seabass (Lates calcarifer) immunised with rHSP90, rHSP33, rHSP70, and rDnaJ showed 48.28%, 62.07%, 51.72%, and 31.03% relative percent survival, respectively, after being challenged with Phdp strain AOD105021. High expression levels of immune-related genes and high antibody titres were observed in the rHSP33 group, and the sera of this group also exhibited a high level of bactericidal activity against Phdp. Collectively, our results suggest that HSP33 is a potential candidate for vaccine development against Phdp infection. |
| Databáze: | OpenAIRE |
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