X-Plate Technology: a new method for detecting fluconazole resistance in Candida species
| Autor: | Scott E. Gygax, Martin E. Adelson, Eli Mordechai, John-Paul Vermitsky, Jessica A. Schuyler, Sean G. Chadwick |
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| Rok vydání: | 2013 |
| Předmět: |
Microbiology (medical)
Antifungal Antifungal Agents medicine.drug_class Cost-Benefit Analysis Colony Count Microbial Agar Dilution Method Antifungal drug Microbial Sensitivity Tests Biology Microbiology Rapid detection Drug Resistance Fungal medicine Humans Species identification Patient treatment Fluconazole Candida General Medicine Culture Media Chromogenic Compounds Vulvovaginal Candidiasis medicine.drug |
| Zdroj: | Journal of Medical Microbiology. 62:720-726 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Candida species are responsible for many opportunistic fungal infections. Fluconazole is a well-tolerated antifungal drug, commonly used in the treatment of candidiasis. However, with fluconazole resistance ever increasing, rapid detection and antifungal susceptibility testing of Candida is imperative for proper patient treatment. This paper reports a cost-effective, simple and rapid chromogenic agar dilution method for simultaneous Candida species identification and fluconazole susceptibility testing. The results obtained by X-Plate Technology were in absolute concordance with standard microbroth dilution assays. Analysis of 1383 clinical patient samples with suspected vulvovaginal candidiasis revealed that this technology was able to detect and speciate the Candida isolate and determine the fluconazole susceptibility. The prevalence and susceptibility profiles of the clinical isolates using this method were highly similar to published reports using the microbroth dilution method. |
| Databáze: | OpenAIRE |
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