Immunohistochemical demonstration of calcitonin binding to specific cell types in fixed rat bone tissue
| Autor: | J. N. M. Heersche, Leticia G. Rao, W. C. Sturtridge, L. L. Marchuk |
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| Rok vydání: | 1981 |
| Předmět: |
Calcitonin
Cell type medicine.medical_specialty Receptors Cell Surface Biology Cross Reactions Bone tissue Peripheral blood mononuclear cell Bone and Bones Immunoenzyme Techniques Endocrinology Internal medicine medicine Calcitonin binding Animals Periosteum Histocytochemistry Immune Sera Receptors Calcitonin Staining Rats medicine.anatomical_structure Animals Newborn Immunohistochemistry |
| Zdroj: | Endocrinology. 108(5) |
| ISSN: | 0013-7227 |
| Popis: | Deparaffinized sections of fixed decalcified radii of 2- to 3-day-old rats were incubated in either salmon calcitonin (sCT; 0.1–1.0 MRC U/ml) or in hormone-solvent and then stained for CT by the peroxidase-antiperoxidase method using rabbit antiserum to sCT and 3,3′-diaminobenzidine-H2O2. The sCT-treated radii showed intense brown staining over the cytoplasm of all identifiable osteoclasts and over the osteoblastic layer of the periosteum. Similar intensity of staining was observed in some osteocytes and some mononuclear cells in the marrow spaces. Striated muscle fibers attached to the radii and erythrocytes were also stained. No staining was observed in the chondrocytes, the osteogenic layer of the periosteum, most osteocytes, any of the endosteal osteoblasts, and the majority of mononuclear cells in the marrow spaces. Cell types in sections of bone that stained strongly after sCT incubation also showed faint staining after incubation with CT-solvent. This observation could be due to the presence of end... |
| Databáze: | OpenAIRE |
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