Gene gun mediated vaccination is superior to manual delivery for immunisation with DNA vaccines expressing protective antigens from Yersinia pestis or Venezuelan Equine Encephalitis virus
| Autor: | Susan C Jacobs, Robert J. Phillpotts, E. Diane Williamson, Stuart D Perkins, Alice M. Bennett |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Pore Forming Cytotoxic Proteins
Injections Intradermal Yersinia pestis Alphavirus Biology medicine.disease_cause Injections Intramuscular complex mixtures Virus Gene gun Microbiology DNA vaccination Encephalitis Virus Venezuelan Equine Mice Plasmid Viral Envelope Proteins Antigen Vaccines DNA medicine Animals Humans Antigens Bacterial Mice Inbred BALB C General Veterinary General Immunology and Microbiology Public Health Environmental and Occupational Health Viral Vaccines DNA Biolistics biology.organism_classification Virology Infectious Diseases Child Preschool Bacterial Vaccines Venezuelan equine encephalitis virus Molecular Medicine Female Gold Plasmids |
| Zdroj: | Vaccine. 18:588-596 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(99)00317-5 |
| Popis: | Plasmids expressing the V antigen of Yersinia pestis or the E2 glycoprotein of Venezuelan Equine Encephalitis (VEE) virus were used to vaccinate mice by intra-dermal or intra-muscular injection, or by particle-mediated bombardment using the Helios™ gene gun. After two immunizations, groups of mice which had received 4 μg doses of plasmid DNA using the gene gun had IgG levels which were higher than in other groups manually immunised with 12-fold more plasmid DNA. The immunoglobulin isotype profile was predominantly IgG1 following inoculation with either plasmid. Our results indicate that gene gun mediated vaccination can be used to increase the magnitude of the immune response to both bacterial and viral antigens expressed by plasmid DNA. |
| Databáze: | OpenAIRE |
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