Functional characterization of the BvgAS two-component system of Bordetella holmesii
| Autor: | Gabriele Gerlach, Roy Gross, Simone Janzen, Dagmar Beier |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
DNA
Bacterial Bordetella pertussis Histidine Kinase Bordetella Virulence Factors Molecular Sequence Data Electrophoretic Mobility Shift Assay Bordetella hinzii Microbiology Bordetella parapertussis Bacterial Proteins Virulence Factors Bordetella Promoter Regions Genetic Regulator gene Bordetella holmesii Bordetella bronchiseptica Base Sequence biology Genetic Complementation Test Gene Expression Regulation Bacterial Sequence Analysis DNA biology.organism_classification Adaptation Physiological Response regulator Bordetella trematum Protein Kinases Gene Deletion Protein Binding Signal Transduction Transcription Factors |
| Zdroj: | Microbiology. 150:3715-3729 |
| ISSN: | 1465-2080 1350-0872 |
| Popis: | The BvgAS two-component system is the master regulator of virulence gene expression in the mammalian pathogens Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. This paper reports the partial cloning and characterization of the bvgAS loci of the ‘new’ Bordetella species Bordetella holmesii, Bordetella trematum and Bordetella hinzii, which are increasingly recognized as opportunistic pathogens in humans. It is demonstrated that the cytoplasmic signalling domains of the BvgS histidine kinases of B. pertussis and B. holmesii are functionally interchangeable, while signal perception by the two sensor proteins seems to be different. Furthermore, it is shown that, despite the high similarity of the BvgA proteins of B. pertussis and B. holmesii, promoter recognition by the response regulator proteins differs substantially in these organisms. |
| Databáze: | OpenAIRE |
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