Rapid and Cost-Effective Genotyping Protocol for Angiotensin-Converting Enzyme Insertion/Deletion (Ins/Del) Polymorphism from Saliva
| Autor: | Miho Imai, Miwako Moritsugu, Atsushi Ichikawa, Shigenori Murata, Kae Fukumoto, Sayuri Kawai, Kenji Kinoshita, Yui Sakaguchi, Madoka Kisoi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Saliva Genotype Genotyping Techniques Pharmaceutical Science Peptidyl-Dipeptidase A Real-Time Polymerase Chain Reaction Melting curve analysis 03 medical and health sciences 0302 clinical medicine Asian People INDEL Mutation Humans Insertion deletion Gene Genotyping Pharmacology Polymorphism Genetic biology Chemistry Angiotensin-converting enzyme General Medicine Molecular biology DNA extraction 030104 developmental biology Real-time polymerase chain reaction 030220 oncology & carcinogenesis biology.protein Female |
| Zdroj: | Biological and Pharmaceutical Bulletin. 42:1345-1349 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.b19-00110 |
| Popis: | DNA extraction and purification have been generally considered to be required for PCR assay. We demonstrated a new protocol using biological specimens directly as templates for real-time PCR with melting curve analysis. We confirmed the melting curve analysis was particularly suitable for the identification of the insertion/deletion (Ins/Del) polymorphism of the angiotensin-converting enzyme (ACE) gene. The new protocol we developed can be set up using simple and complete PCR analysis including data interpretation in under four hours with additional advantages of application for large-scale clinical research, diagnostics, and epidemiological studies at low cost. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|