Diagnostic and prognostic role of urinary collagens in primary human bladder cancer

Autor: Shunta Hori, Yoshihiro Tatsumi, Noboru Konishi, Kota Iida, Makito Miyake, Yasushi Nakai, Kenta Onishi, Yosuke Morizawa, Keiji Shimada, Sayuri Onishi, Kazumasa Torimoto, Takuya Owari, Nobumichi Tanaka, Satoshi Anai, Katsuya Aoki, Kiyohide Fujimoto, Daisuke Gotoh, Yoshitomo Chihara
Rok vydání: 2017
Předmět:
Collagen Type IV
Male
0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
recurrence
Urinary system
Human bladder
Urology
Alpha (ethology)
Enzyme-Linked Immunosorbent Assay
Urine
03 medical and health sciences
0302 clinical medicine
Antigens
Neoplasm
Clinical Research
Biomarkers
Tumor
medicine
Humans
Neoplasm Invasiveness
Risk factor
Aged
Glycoproteins
Urine cytology
Keratin-19
Bladder cancer
medicine.diagnostic_test
business.industry
urinary bladder neoplasm
Nuclear Proteins
Cancer
Original Articles
General Medicine
Middle Aged
Prognosis
medicine.disease
urine
Gene Expression Regulation
Neoplastic
enzyme‐linked immunosorbent assay
030104 developmental biology
Urinary Bladder Neoplasms
Oncology
030220 oncology & carcinogenesis
Female
Original Article
Collagen
Neoplasm Recurrence
Local
business
Zdroj: Cancer Science
ISSN: 1349-7006
1347-9032
DOI: 10.1111/cas.13384
Popis: Collagen type 4 alpha 1 (COL4A1) and collagen type 13 alpha 1 (COL13A1) produced by urothelial cancer cells support the vital oncogenic property of tumor invasion. We investigated the diagnostic and prognostic capability of COL4A1 and COL13A1 in voided urine and compared the observed values with those of fragments of cytokeratin‐19 (CYFRA21‐1), nuclear matrix protein 22 (NMP‐22), and voided urine cytology in bladder cancer (BCa). We collected voided urine samples from 154 patients newly diagnosed with BCa, before surgery and from 61 control subjects. Protein levels of COL4A1, COL13A1, CYFRA21‐1, and NMP‐22 in urine supernatants were measured using enzyme‐linked immunosorbent assays. Diagnostic performance and optimal cut‐off values were determined by receiver operating characteristic analysis. Urine levels of COL4A1, COL13A1, the combined values of COL4A1 and COL13A1 (COL4A1 + COL13A1), and CYFRA21‐1 were significantly elevated in urine from patients with BCa compared to the controls. Among these biomarkers, the optimal cut‐off value of COL4A1 + COL13A1 at 1.33 ng/mL resulted in 57.4%, 83.7%, 56.1%, 80.7%, and 91.7% sensitivity for low‐grade tumors, high‐grade tumors, Ta, T1, and muscle invasive disease, respectively. We evaluated the prognostic value of preoperative urine levels in 130 non‐muscle invasive BCa samples after the initial transurethral surgery. A high urinary COL4A1 + COL13A1 was found to be an independent risk factor for intravesical recurrence. Although these data need to be externally validated, urinary COL4A1 and COL13A1 could be a potential diagnostic and prognostic biomarker for BCa. This easy‐to‐use urinary signature identifies a subgroup of patients with a high probability of recurrence and progression in non‐muscle invasive and muscle invasive BCa.
Databáze: OpenAIRE
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