The cytotoxicity and antioxidant activity analysis of the isolated constituents and extracts from endemic Centaurea derderiifolia
| Autor: | Lütfi Behçet, Feyza Oke-Altuntas, Ibrahim Demirtas, Fatma Bulut, Tevfik Ozen, Serkan Koldas, Fatih Gul |
|---|---|
| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
medicine.medical_treatment Plant Science 01 natural sciences Biochemistry HeLa Analytical Chemistry daucosterin medicine Cytotoxic T cell Cytotoxicity Centaurea derderiifolia radical scavenging biology Traditional medicine 010405 organic chemistry Chemistry Cervical adenocarcinoma Organic Chemistry food and beverages biology.organism_classification NMR 0104 chemical sciences 010404 medicinal & biomolecular chemistry Centaurea |
| Zdroj: | Natural Product Research. 35:1919-1922 |
| ISSN: | 1478-6427 1478-6419 |
| Popis: | gul, fatih/0000-0002-4297-786X; Koldas, Serkan/0000-0002-7917-4008; demirtas, ibrahim/0000-0001-8946-647X WOS: 000477221000001 PubMed: 31334668 It is aimed to investigate the cytotoxic effects of the various extracts from the leaf and seed of Centaurea derderiifolia on the growth human cervical adenocarcinoma (HeLa) cells by xCELLigence method and to isolate the cytotoxic constituents. The results showed that subfractions 2 and 3 exhibited remarkable inhibitory effect against HeLa (IC50 < 10 mu g/mL). The chloroform extract of leaf displayed the highest cytotoxic effect on HeLa cells (IC50 < 50 mu g/mL) and was therefore subjected to a bioassay-guided multistep separation procedure. The pure compounds were elucidated by spectroscopic and mass-spectrometric analyses, including 1 D-, 2 D-NMR. In addition to cytotoxic effects of the isolated constituents, their antioxidant activities were also studied. On the other hand, subfraction 4 exhibited the highest DPPH radical scavenging activity (IC50 = 0.76 +/- 0.03 mg/mL). beta-sitosterol-3-O-beta-d-glucopyranoside was isolated for the first time from this plant and three compounds from the bioactive subfractions were identified. Cankiri Karatekin UniversityCankiri Karatekin University [BAP: 2013-20] The authors would like to thank Merve B. Koc for her help to researcher in the xCELLigence tests and Cankiri Karatekin University for financial support (BAP: 2013-20). |
| Databáze: | OpenAIRE |
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