AtC3H17, a Non-Tandem CCCH Zinc Finger Protein, Functions as a Nuclear Transcriptional Activator and Has Pleiotropic Effects on Vegetative Development, Flowering and Seed Development in Arabidopsis
| Autor: | Hye-Yeon Seok, Sun-Young Lee, Dong-Hyuk Woo, Eun-Hye Lee, Huong T. Tran, Linh Vu Nguyen, Yong-Hwan Moon, Hee-Yeon Park |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transcriptional Activation Physiology Mutant Amino Acid Motifs Arabidopsis Glutamic Acid Germination Plant Science Flowers Genes Plant 01 natural sciences 03 medical and health sciences Transactivation Protein Domains Gene Expression Regulation Plant Amino Acid Sequence Promoter Regions Genetic Gene Conserved Sequence Zinc finger Genetics Cell Nucleus biology Arabidopsis Proteins fungi Wild type food and beverages Genetic Pleiotropy Zinc Fingers Cell Biology General Medicine biology.organism_classification Cell biology 030104 developmental biology Phenotype Seedling Organ Specificity Mutation Seeds Trans-Activators 010606 plant biology & botany |
| Zdroj: | Plantcell physiology. 57(3) |
| ISSN: | 1471-9053 |
| Popis: | Despite increasing reports that CCCH zinc finger proteins function in plant development and stress responses, the functions and molecular aspects of many CCCH zinc finger proteins remain uncharacterized. Here, we characterized the biological and molecular functions of AtC3H17, a unique Arabidopsis gene encoding a non-tandem CCCH zinc finger protein. AtC3H17 was ubiquitously expressed throughout the life cycle of Arabidopsis plants and their organs. The rate and ratio of seed germination of atc3h17 mutants were slightly slower and lower, respectively, than those of the wild type (WT), whereas AtC3H17-overexpressing transgenic plants (OXs) showed an enhanced germination rate. atc3h17 mutant seedlings were smaller and lighter than WT seedlings while AtC3H17 OX seedlings were larger and heavier. In regulation of flowering time, atc3h17 mutants showed delayed flowering, whereas AtC3H17 OXs showed early flowering compared with the WT. In addition, overexpression of AtC3H17 affected seed development, displaying abnormalities compared with the WT. AtC3H17 protein was localized to the nucleus and showed transcriptional activation activity in yeast and Arabidopsis protoplasts. The N-terminal region of AtC3H17, containing a conserved EELR-like motif, was necessary for transcriptional activation activity, and the two conserved glutamate residues in the EELR-like motif played an important role in transcriptional activation activity. Real-time PCR and transactivation analyses showed that AtC3H17 might be involved in seed development via transcriptional activation of OLEO1, OLEO2 and CRU3. Our results suggest that AtC3H17 has pleiotropic effects on vegetative development such as seed germination and seedling growth, flowering and seed development, and functions as a nuclear transcriptional activator in Arabidopsis. |
| Databáze: | OpenAIRE |
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