Micelles self-assembled by 3-O-β-d-glucopyranosyl latycodigenin enhance cell membrane permeability, promote antibiotic pulmonary targeting and improve anti-infective efficacy
| Autor: | Wenbo Wu, Dandan Cheng, Zhi-Hong Jiang, Lili Ye, Yuanyuan Hou, Fukui Shen, Man Zhang, Hao Huang, Kai-Xin Liu, Gang Bai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Drug
Male lcsh:Medical technology media_common.quotation_subject lcsh:Biotechnology Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Pharmacology Lung injury Applied Microbiology and Biotechnology Micelle chemistry.chemical_compound Mice Drug Delivery Systems In vivo Lactate dehydrogenase Cell Line Tumor lcsh:TP248.13-248.65 Distribution (pharmacology) Animals Humans Particle Size Lung Micelles media_common Drug Carriers Self-assembled nanomicelles Chemistry Research Saponins Anti-Bacterial Agents Cell membrane permeability lcsh:R855-855.5 Critical micelle concentration Drug delivery Molecular Medicine Cytokines Nanoparticles 3-O-β-d-glucopyranosyl latycodigenin Lung-targeting therapy |
| Zdroj: | Journal of Nanobiotechnology, Vol 18, Iss 1, Pp 1-15 (2020) Journal of Nanobiotechnology |
| ISSN: | 1477-3155 |
| Popis: | Background Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. They exhibit significant advantages compared to traditional pulmonary drug delivery systems. However, developing the formulation of each drug is a time-consuming and laborious task. Results In this study, a universal lung-targeting nanoparticle was designed and constructed. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-d-glucopyranosyl platycodigenin 682 (GP-682), based on its specific amphiphilic structure. The GP-682 micelles exhibited a relatively stable zeta potential with a particle size between 60 and 90 nm, and the critical micelle concentration (CMC) value was approximately 42.3 μg/mL. Preincubation of GP-682 micelles markedly enhanced their cell membrane permeability and improved drug uptake in vitro. The results were visualized using fluorescent dye tracing, transmission electron microscopy (TEM) observations and the lactate dehydrogenase (LDH) release assay. The obtained benefits enhanced the distribution of levofloxacin (Lev) in mouse lung tissue and reduced antibiotics overdosing. The acute lung injury mouse model induced by the Pseudomonas aeruginosa PA 14 strain demonstrated that preinjection of GP-682 micelles before antibiotic administration resulted in a higher survival rate and anti-infective efficacy in vivo. It also caused reductions in pulmonary injury, bacterial invasion and cytokine expression compared with treatment with Lev alone. Conclusions GP-682 micelles are another nanoparticle-based pulmonary drug delivery system and provide a new lung-targeting therapy option. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |