Expression and significance of telomerase in the nucleus pulposus tissues of degenerative lumbar discs
| Autor: | Xu Zhang, Ming‑Kun Yang, Jie Wang, Zhou Li, Chuan Liu, Ji‑Sheng Wu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Telomerase
Pathology medicine.medical_specialty General Neuroscience Cell Type II collagen General Medicine Articles Biology Molecular medicine General Biochemistry Genetics and Molecular Biology Pathogenesis chemistry.chemical_compound Lumbar medicine.anatomical_structure chemistry medicine Immunohistochemistry Chondroitin sulfate General Pharmacology Toxicology and Pharmaceutics |
| Zdroj: | Biomedical reports. 3(6) |
| ISSN: | 2049-9434 |
| Popis: | The pathogenesis of lumbar disc degeneration is extremely complex, and the expression and role of telomerase in degenerative lumbar disc tissues remains unclear. The aim of the present study was to detect telomerase expression in nucleus pulposus tissues of degenerative lumbar discs and to explore the correlation between telomerase expression and other factors typical of disc degeneration. A total of 8 patients with degenerative nucleus pulposus were included as the experimental group and compared with 8 control patients without evident lumbar disc degeneration. The expression of telomerase in nucleus pulposus tissues was detected by immunohistochemical staining. ELISA was performed to determine the differential expression of telomerase, type II collagen and chondroitin sulfate between the two groups. In addition, a correlation analysis was performed to form associations between these factors. Finally, 5 cases in the experimental group and 5 in the control group were involved in the analysis. Immunohistochemistry results showed that telomerase expression in the experimental group was significantly lower compared to the control group and the percentage in the unit field of view showed significant differences between the two groups (P |
| Databáze: | OpenAIRE |
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