Gene expression profiling of monocytes displaying herpes simplex virus 1 induced dysregulation of antifungal defences
| Autor: | Carlotta Francesca Orsi, Elisabetta Blasi, Andrea Ardizzoni, Samuele Peppoloni, Claudio Cermelli, A. Cuoghi, Enrico Tagliafico, Rachele Giovanna Neglia |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Phagocytosis HSV-1 Candida albicans monocytes phagocytosis antimicrobial activity gene expression Apoptosis Herpesvirus 1 Human medicine.disease_cause Microbiology Monocytes Virus Cell Line Chlorocebus aethiops Cell Adhesion medicine Animals Humans Vero Cells Oligonucleotide Array Sequence Analysis Respiratory Burst biology Gene Expression Profiling Monocyte General Medicine Macrophage Activation Opsonin Proteins biology.organism_classification Respiratory burst Gene expression profiling medicine.anatomical_structure Herpes simplex virus Host-Pathogen Interactions |
| Zdroj: | Journal of Medical Microbiology. 58:1283-1290 |
| ISSN: | 1473-5644 0022-2615 |
| DOI: | 10.1099/jmm.0.011023-0 |
| Popis: | Recently, we showed that herpes simplex virus 1 (HSV-1)-infected monocytes have altered antifungal defences, in particular they show augmented phagocytosis of Candida albicans followed by a failure of the intracellular killing of the ingested fungi. On the basis of these functional data, comparative studies were carried out on the gene expression profile of cells infected with HSV-1 and/or C. albicans in order to investigate the molecular mechanisms underlying such virus-induced dysfunction. Affymetrix GeneChip technology was used to evaluate the cell transcription pattern, focusing on genes involved in phagocytosis, fungal adhesion, antimicrobial activity and apoptosis. The results indicated there was: (a) prevalent inhibition of opsonin-mediated phagocytosis, (b) upregulation of several pathways of antibody- and complement-independent phagocytosis, (c) inhibition of macrophage activation, (d) marked dysregulation of oxidative burst, (e) induction of apoptosis. |
| Databáze: | OpenAIRE |
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