Tissue-specific expression of IgG receptors by human macrophages ex vivo
| Autor: | Thomas M. van Gulik, Barbara Dierdorp, Jesper Kers, Christine W. Bruggeman, Joke M. M. den Haan, René Lutter, Timo K. van den Berg, Julia H E Houtzager, Robin van Bruggen, Taco W. Kuijpers, Steven T. Pals |
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| Přispěvatelé: | Molecular cell biology and Immunology, AII - Inflammatory diseases, Pediatric surgery, APH - Aging & Later Life, Amsterdam Gastroenterology Endocrinology Metabolism, Pathology, Pulmonology, Surgery, AGEM - Endocrinology, metabolism and nutrition, AGEM - Re-generation and cancer of the digestive system, General Internal Medicine, Landsteiner Laboratory, Paediatric Infectious Diseases / Rheumatology / Immunology, Amsterdam Reproduction & Development (AR&D), APH - Digital Health, APH - Global Health |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Physiology Liver cytology Monocytes White Blood Cells Spectrum Analysis Techniques 0302 clinical medicine Animal Cells Immune Physiology Medicine and Health Sciences Macrophage Alveolar Macrophages Receptor Lung Multidisciplinary medicine.diagnostic_test Chemistry Flow Cytometry Body Fluids Cell biology Blood medicine.anatomical_structure Liver Spectrophotometry Organ Specificity Medicine Cytophotometry Cellular Types Anatomy Research Article Kupffer Cells Immune Cells Phagocytosis Science Immunology Bone Marrow Cells Spleen Research and Analysis Methods Flow cytometry 03 medical and health sciences medicine Humans Molecular Biology Techniques Molecular Biology Blood Cells Macrophages Receptors IgG Biology and Life Sciences Cell Biology 030104 developmental biology Bone marrow Ex vivo Cloning 030215 immunology |
| Zdroj: | Bruggeman, C W, Houtzager, J, Dierdorp, B, Kers, J, Pals, S T, Lutter, R, van Gulik, T, den Haan, J M M, van den Berg, T K, van Bruggen, R & Kuijpers, T W 2019, ' Tissue-specific expression of IgG receptors by human macrophages ex vivo ', PLoS ONE, vol. 14, no. 10, e0223264 . https://doi.org/10.1371/journal.pone.0223264 PLoS ONE, 14(10):e0223264. Public Library of Science PLoS ONE, Vol 14, Iss 10, p e0223264 (2019) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0223264 |
| Popis: | Recently it was discovered that tissue-resident macrophages derive from embryonic precursors, not only from peripheral blood monocytes, and maintain themselves by self-renewal. Most in-vitro studies on macrophage biology make use of in-vitro cultured human monocytederived macrophages. Phagocytosis of IgG-opsonized particles by tissue-resident macrophages takes place via interaction with IgG receptors, the Fc-gamma receptors (FcγRs). We investigated the FcγR expression on macrophages both in-vivo and ex-vivo from different human tissues. Upon isolation of primary human macrophages from bone marrow, spleen, liver and lung, we observed that macrophages from all studied tissues expressed high levels of FcγRIII, which was in direct contrast with the low expression on blood monocyte- derived macrophages. Expression levels of FcγRI were highly variable, with bone marrow macrophages showing the lowest and alveolar macrophages the highest expression. Kupffer cells in the liver were the only tissue-resident macrophages that expressed the inhibitory IgG receptor, FcγRIIB. This inhibitory receptor was also found to be expressed by sinusoidal endothelial cells in the liver. In sum, our immunofluorescence data combined with ex-vivo stainings of isolated macrophages indicated that tissue-resident macrophages are remarkably unique and different from monocyte-derived macrophages in their phenotypic expression of IgG receptors. Tissue macrophages show distinct tissue-specific FcγR expression patterns. |
| Databáze: | OpenAIRE |
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