Transferability, development of Single Sequence Repeat (SSR) markers and application to the analysis of genetic diversity and population structure of the African fan palm (Borassus aethiopumMart.) in Benin
| Autor: | Valère Kolawolé Salako, Kifouli Adeoti, Christine Tollon-Cordet, Thierry Beulé, Leila Zekraoui, Sylvain Santoni, Mariano Joly Kpatenon, Muriel Latreille, Estelle Jaligot, Cédric Mariac |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences education.field_of_study Genetic diversity biology Population Transferability Population structure 15. Life on land biology.organism_classification 01 natural sciences Borassus aethiopum Fixation index 03 medical and health sciences Evolutionary biology Microsatellite Palm education 030304 developmental biology 010606 plant biology & botany |
| DOI: | 10.1101/2020.01.30.926626 |
| Popis: | In Sub-Saharan Africa, the fan palmBorassus aethiopumMart. is an important non-timber forest product-providing palm that faces multiple anthropogenic threats to its genetic diversity. However, this species is so far under-studied, which prevents its sustainable development as a resource.The present work is a first attempt at characterizing the genetic diversity of this palm species as well as its spatial structuration in Benin, West Africa. During a first phase we implemented a microsatellite markers-based approach relying on the reported transferability of primers developed in other palm species and found that, in disagreement with previously published results, only 22.5% of the 80 markers tested enabled amplification of African fan palm DNA and polymorphism detection was insufficient. During a second phase, we therefore generated aB. aethiopum-specific genomic dataset through high-throughput sequencing and used it for thede novodetection of potential microsatellite markers. Among these, 11 enabled polymorphism detection and were further used for analyzing genetic diversity in nineB. aethiopumpopulations.Our results show that genetic diversity of Beninese fan palm populations is low, with an overall average expected heterozygosity (He) of 0.354. Moreover, the positive values of the fixation index (F) in populations from both the Central (Soudano-Guinean) and the Southern (Guinean) regions suggest limited gene flows. Our analysis show that sampledB. aethiopumpopulations are clustered into two groups, one spanning populations from both the Southern and most of the Central region, and the other including the Central population of Savè (which also has the highest He) and populations from the North.In light of our results, we discuss the use of inter-species transfer vs.de novodevelopment of microsatellite markers in genetic diversity analyses targeting under-studied species. We also suggest future applications for the molecular resources generated through the present study. |
| Databáze: | OpenAIRE |
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