Adipose-derived stem cells express higher levels of type VII collagen under specific culture conditions
| Autor: | Hideo Iida, Toshio Hasegawa, Akino Wada, Tatsuo Fukai, Shigaku Ikeda, Atsushi Sakamoto, Yuichiro Maeda |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Keratinocytes Collagen Type VII Cellular differentiation Population Down-Regulation Dermatology Biology 030207 dermatology & venereal diseases 03 medical and health sciences Type IV collagen 0302 clinical medicine medicine Adipocytes Humans Progenitor cell education Cells Cultured Dermoepidermal junction education.field_of_study Wound Healing Stem Cells Keratin-14 Cell Differentiation General Medicine Fibroblasts Keratin-10 Flow Cytometry Molecular biology Coculture Techniques 030104 developmental biology medicine.anatomical_structure Adipose Tissue Epidermal Cells Stem cell Epidermis Keratinocyte Wound healing |
| Zdroj: | Archives of dermatological research. 309(10) |
| ISSN: | 1432-069X |
| Popis: | Type VII collagen (Col7) is a major component of the anchoring fibrils at the dermoepidermal junction. Adipose-derived stem cells (ADSCs) are a cell population highly useful in regenerative medicine because of their ease of isolation and their potential for multilineage differentiation. Based on the observations that K14 was expressed in undifferentiated ADSCs and the expression was downregulated after differentiation into adipocytes, we speculated that ADSCs are keratinocyte stem/progenitor cells. ADSCs were co-cultured with fibroblasts on type IV collagen in a medium containing all-trans retinoic acid and bone morphogenetic protein 4. At day 14 of culture in keratinocyte serum-free medium, the cells were harvested and subjected to immunofluorescence, flow cytometry, real-time PCR, and western blotting. Approximately, 45% of ADSCs were immunostained positively for anti-human cytokeratin 10, and approximately 80% were stained positively for Col7. Flow cytometry, real-time PCR, and western blotting also confirmed that differentiated ADSCs expressed higher levels of Col7. These findings support the therapeutic potential of ADSCs, not only for wound healing, but also for the correction of Col7 deficiencies. |
| Databáze: | OpenAIRE |
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