Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
| Autor: | Calvin Simerly, Jocelyn D. Mich-Basso, Christopher S. Navara, Stacie Oliver, Carlos A. Castro, Carrie J. Redinger, Ahmi Ben-Yehudah |
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| Rok vydání: | 2007 |
| Předmět: |
Pluripotent Stem Cells
Primates Homeobox protein NANOG Transplantation Heterologous Cell Cell Culture Techniques Gene Expression Zoology Cell Separation Mice SCID Biology Oct-4 Mice Mice Inbred NOD medicine Animals Embryonic Stem Cells DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction Teratoma Cell Biology General Medicine medicine.disease Immunohistochemistry Embryonic stem cell Phenotype In vitro Cell biology medicine.anatomical_structure Karyotyping Stem cell Developmental Biology |
| Zdroj: | Current Protocols in Stem Cell Biology |
| ISSN: | 1938-8969 1941-7322 |
| DOI: | 10.1002/9780470151808.sc01a01s1 |
| Popis: | Embryonic stem (ES) cells are a powerful research tool enabling the generation of mice with custom genetics, the study of the earliest stages of mammalian differentiation in vitro and, with the isolation of human ES cells, the potential of cell-based therapies for a number of diseases including Parkinson's and Type 1 diabetes. ES cells isolated from nonhuman primates (nhpES cells) offer the opportunity to ethically test the developmental potential of primate ES cells in chimeric offspring. If these cells have similar potency to mouse ES cells, this may open a new era of primate models of human disease. Nonhuman primates are the perfect model system for the preclinical testing of ES cell–derived therapies. In this unit, we describe methods for the derivation and characterization of nonhuman primate ES cells. With these protocols, the investigator will be able to isolate nhpES cells and perform the necessary tests to confirm the pluripotent phenotype. Curr. Protoc. Stem Cell Biol. 1:1A.1.1-1A.1.21. © 2007 by John Wiley & Sons, Inc. Keywords: nonhuman primate; embryonic stem cells; Oct-4; Nanog; Karyotype; Teratoma |
| Databáze: | OpenAIRE |
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