Correlation of transepidermal water loss with skin barrier propertiesin vitro: comparison of three evaporimeters
| Autor: | Lilian Tian, Rania Elkeeb, Heidi Chan, Xiaoying Hui, Howard I. Maibach |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Skin barrier Tritiated water Skin Absorption Analytical chemistry Human skin Dermatology In Vitro Techniques Tritium Young Adult chemistry.chemical_compound medicine Humans Radionuclide Imaging Diffusion cell Aged Transepidermal water loss Chromatography Chemistry Water Middle Aged Permeation Water Loss Insensible medicine.anatomical_structure Absorbed dose Epidermis Skin Temperature |
| Zdroj: | Skin Research and Technology. 16:9-15 |
| ISSN: | 1600-0846 0909-752X |
| Popis: | Aim: This study investigates the relationship between transepidermal water loss (TEWL) and skin permeability to tritiated water as a rapid assessment of the integrity of the barrier properties of skin as part of in vitro skin permeation studies. Methods: TEWL values before and during the experimental period were measured using three evaporimeters (A, B, and C) representing different measuring principles and technologies. Single application of tritiated water was dosed on dermatomed human skin samples in a flow-through diffusion cell system. Radioactivity of the absorbed dose and the removable dose residues was counted to determine percent dose and flux rate. These data were further combined with TEWL values to analyze the correlation. Results: Evaporimeter C, a closed chamber–condenser technology, had higher measurement capacity than other instruments, evaporimeter A, an open chamber, and evaporimeter B, a closed chamber (P |
| Databáze: | OpenAIRE |
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