Cloning of the human sodium lodide symporter
| Autor: | Tara L. Furminger, Shunhua Xing, Qing-Rong Liu, Ernest L. Mazzaferri, Patricia A. Smanik, Sissy M. Jhiang, Kwon-Yul Ryu |
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| Rok vydání: | 1996 |
| Předmět: |
Sodium-iodide symporter
endocrine system DNA Complementary endocrine system diseases Molecular Sequence Data Biophysics Biochemistry Cell Line Thyroid hormone receptor beta Thyroid carcinoma Thyroid peroxidase medicine Tumor Cells Cultured Animals Humans Iodide transport Amino Acid Sequence Thyroid Neoplasms Cloning Molecular Molecular Biology Thyroid cancer Thyroid hormone receptor Perchlorates biology Base Sequence Sequence Homology Amino Acid Symporters Chemistry Thyroid Membrane Proteins Cell Biology Iodides medicine.disease Molecular biology Rats medicine.anatomical_structure biology.protein Carrier Proteins |
| Zdroj: | Biochemical and biophysical research communications. 226(2) |
| ISSN: | 0006-291X |
| Popis: | The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity. |
| Databáze: | OpenAIRE |
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