A knockout screen of genes expressed specifically in Ae. aegypti pupae reveals a critical role for stretchin in mosquito flight
| Autor: | Collin Valentin, Zach N. Adelman, Kevin M. Myles, Keun Chae, Chanell Dawson, Emma Jakes |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Mosquito Control animal structures Protein family Transgene Vector Borne Diseases Genes Insect Mosquito Vectors Aedes aegypti Biology medicine.disease_cause 01 natural sciences Biochemistry Marker gene Animals Genetically Modified 03 medical and health sciences Aedes Gene expression medicine Animals Drosophila Proteins Chikungunya Myosin-Light-Chain Kinase Molecular Biology Gene Gene knockout 030304 developmental biology Genetics 0303 health sciences fungi Pupa biology.organism_classification 010602 entomology Flight Animal Gene Knockdown Techniques Insect Science Insect Proteins |
| Zdroj: | Insect Biochemistry and Molecular Biology. 132:103565 |
| ISSN: | 0965-1748 |
| DOI: | 10.1016/j.ibmb.2021.103565 |
| Popis: | Aedes aegypti is a critical vector for transmitting Zika, dengue, chikungunya, and yellow fever viruses to humans. Genetic strategies to limit mosquito survival based upon sex distortion or disruption of development may be valuable new tools to control Ae. aegypti populations. We identified six genes with expression limited to pupal development ; osi8 and osi11 (Osiris protein family), CPRs and CPF (cuticle protein family), and stretchin (a muscle protein). Heritable CRISPR/Cas9-mediated gene knockout of these genes did not reveal any defects in pupal development. However, stretchin-null mutations (strnΔ35/Δ41) resulted in flightless mosquitoes with an abnormal open wing posture. The inability of adult strnΔ35/Δ41 mosquitoes to fly restricted their escape from aquatic rearing media following eclosion , and substantially reduced adult survival rates. Transgenic strains which contain the EGFP marker gene under the control of strn regulatory regions (0.8 kb, 1.4 kb, and 2.2 kb upstream, respectively), revealed the gene expression pattern of strn in muscle-like tissues in the thorax during late morphogenesis from L 4 larvae to young adults. We demonstrated that Ae. aegypti pupae-specific strn is critical for adult mosquito flight capability and a key late-acting lethal target for mosquito-borne disease control. |
| Databáze: | OpenAIRE |
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