Production of IL-13 in spleen cells by IL-18 and IL-12 through generation of NK-like cells
| Autor: | Haruyasu Ueda, Atsuo Sekiyama, Haruki Okamura, Naomi Gamachi, Takeharu Ogura, Shin-ichiro Kashiwamura |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
CD8 Antigens
Immunology Population Spleen Cell Separation Biology Biochemistry CD49b Antibodies Interferon-gamma Mice T-Lymphocyte Subsets medicine Immunology and Allergy Animals IL-2 receptor education Molecular Biology education.field_of_study Interleukin-13 Interleukin-18 Granulocyte-Macrophage Colony-Stimulating Factor Receptors Interleukin-2 Hematology Molecular biology Interleukin-12 Rats Killer Cells Natural Mice Inbred C57BL medicine.anatomical_structure Interleukin 13 CD4 Antigens Interleukin 12 Interleukin 18 CD8 |
| Zdroj: | Cytokine. 33(4) |
| ISSN: | 1043-4666 |
| Popis: | Treatment of Nylon wool-passed cells (NWC) prepared from the spleen of C57BL/6 mice with IL-18 and IL-12, but not with IL-18 alone, resulted in induction of IFN-gamma, a Th1 cytokine, and GM-CSF at 24 h, and IL-13, a Th2 cytokine at 72 h. The induction of IL-13 was suppressed by anti-GM-CSF antibody, indicating involvement of GM-CSF in IL-13 production. When NWC incubated with IL-18 and IL-12 for 72 h ("primary treatment") were treated again with the same cytokines ("secondary treatment"), IL-13 was induced much more quickly than observed in the primary treatment. Flow cytometric analysis of NWC after the primary treatment showed marked increases in the CD4(-)CD8(-) non-T cell population bearing CD25(+), CD45RB(super high) and CD122(+). These cells were positive for CD49b but negative for NK1.1, indicating that they were not typical but NK-like cells. The NK-like cells produced IL-13 in response to the treatment with IL-18 alone, indicating that the generation of these cells in the primary treatment likely accounts for the quick production of IL-13 in the secondary treatment. These results show that IL-18 and IL-12 generates the NK-like cells in NWC by a process mediated by GM-CSF that are ready for producing IL-13. |
| Databáze: | OpenAIRE |
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