Effects of laccase on lignin depolymerization and enzymatic hydrolysis of ensiled corn stover
| Autor: | Megan N. Marshall, Qin Chen, Tom L. Richard, Scott M. Geib, Ming Tien |
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| Rok vydání: | 2012 |
| Předmět: |
Environmental Engineering
Glycoside Hydrolases Bioengineering Lignin Zea mays complex mixtures Gas Chromatography-Mass Spectrometry Polymerization chemistry.chemical_compound Cellulase Enzymatic hydrolysis Hemicellulose Food science Cellulose Waste Management and Disposal Stover Trametes Waste Products Laccase Silage Renewable Energy Sustainability and the Environment Hydrolysis food and beverages General Medicine Quaternary Ammonium Compounds Corn stover chemistry Agronomy Cellulosic ethanol Carbohydrate Metabolism |
| Zdroj: | Bioresource Technology. 117:186-192 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2012.04.085 |
| Popis: | The aim of this study was to explore the synergies of laccase, a ligninolytic enzyme, with cellulose and hemicellulase amendments on ensiled corn stover. Molecular signals of lignin decomposition were observed by tetramethylammonium hydroxide thermochemolysis and gas chromatography-mass spectroscopy (TMAH-GC-MS) analysis. The significant findings suggest that ensilage might provide a platform for biological pretreatment. By partially hydrolyzing cellulose and hemicellulose into soluble sugars, ensilage facilitates laccase penetration into the lignocellulose complex to enhance lignin degradation. Downstream cellulose hydrolysis was improved 7% with increasing laccase loading rate. These results demonstrate the potential of enzymes, either directly amended or expressed by microbes during ensilage, to maximize utilization of corn stover for cellulosic biofuels and other downstream fermentations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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