Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by 'SLRP'
| Autor: | Salah A. Sheweita, Ahmed J. Neama, Emad A. Hashish, Amara A. Amro, Ahmed Hussein |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Salmonella typhimurium Salmonella Article Subject medicine.medical_treatment lcsh:Medicine medicine.disease_cause Cell Fractionation lcsh:Technology General Biochemistry Genetics and Molecular Biology Microbiology Rats Sprague-Dawley Antigen medicine Animals lcsh:Science General Environmental Science Antigens Bacterial biology lcsh:T Cell Membrane Vaccination lcsh:R O Antigens Reproducibility of Results General Medicine Virology Antibodies Bacterial Bacterial vaccine Agglutination (biology) Specific antibody Microscopy Electron Antigens Surface Bacterial Vaccines biology.protein lcsh:Q Reagent Kits Diagnostic Antibody Adjuvant Research Article |
| Zdroj: | The Scientific World Journal, Vol 2014 (2014) The Scientific World Journal |
| ISSN: | 2356-6140 |
| Popis: | Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody forSalmonellais available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with correct surface antigens could interact with their specific antibodies. Additionally the study has included oral vaccination with STGs with challenge test. The rats serums have been evaluated against both of the O and H antigens. The antigen-antibody interaction (agglutination) results of both the SLRP and the animal experiments prove that we have correct STGs able to immunize the rats against viableSalmonella. STGs could be used as vaccine and as adjuvant and in the antibodies and in the diagnostic kits production. This study is an additional step for the establishment of correct BGs for immunological purposes. |
| Databáze: | OpenAIRE |
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