A recombinant rat vascular AT1 receptor confers growth properties to angiotensin II in Chinese Hamster Ovary cells
| Autor: | Eric Clauser, Claire Bihoreau, Pierre Corvol, Kenneth E. Bernstein, T. J. Murphy, Catherine Monnot, Betty Teutsch, R. Wayne Alexander |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Angiotensin receptor
Inositol Phosphates Biophysics Hamster CHO Cells Biology Biochemistry chemistry.chemical_compound Cricetinae Animals Insulin Inositol Inositol phosphate Receptor Molecular Biology chemistry.chemical_classification Receptors Angiotensin Angiotensin II receptor type 1 Angiotensin II Chinese hamster ovary cell DNA Cell Biology Molecular biology Recombinant Proteins Rats chemistry Type C Phospholipases Cell Division Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 187:1381-1388 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(92)90455-t |
| Popis: | A rat vascular AT1 receptor cDNA has been stably expressed into Chinese Hamster Ovary cells and the resulting recombinant AT1a receptor has been functionally characterized. This receptor binds 125I Sar1-angiotensin II with an affinity of 0.9 nM and the displacement of this ligand by a series of peptidic and nonpeptidic analogs is shown. Binding of angiotensin II to this receptor causes a rapid increase in inositol phosphate production, whereas this effect is not observed in nontransfected cells. Des-aspartyl1 angiotensin II and at a lesser extent angiotensin I are also able to produce an increase in inositol phosphates. More importantly, the actions of angiotensin II on cell division were clearly demonstrated in this model, since angiotensin II is able to stimulate DNA synthesis by 400% and double the cell population of the transfected cells in 36 hours in the absence of any other growth factor, whereas no effect is observed in nontransfected cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|